This review considers the potential for DNA strand breaks, particularly as measured by the comet assay, to act as a biomarker of genetic toxicity in fish and other aquatic species. The background need for such biomarkers is introduced in relation to carcinogenicity, reproductive effects and other adverse effects of pollution. Sensitive measurements of DNA strand breakage can be achieved, e.g., by alkaline elution, alkaline unwinding or by single cell gel electrophoresis (comet) techniques. The DNA damage can be a reflection, not only of direct strand breakage, but also of alkali-labile sites and of repair enzyme-mediated breakage (i.e., is non-specific). A range of genotoxic chemicals (both with and without the requirement for metabolic activation) give positive effects in various eel; types of vertebrate and invertebrate aquatic species, following in vitro and in vivo exposures under laboratory conditions. A limited number of analyses of organisms exposed to polluted waters or sediments in the field have implicated DNA strand breakage as a relatively sensitive, rapid and broad specificity indicator of genotoxic pollutant exposure. The comet assay deserves f'urther exploitation to assess inter-individual and inter-cell variability in response to pollutants and naturally occurring genotoxic stimuli, and to assess the persistence of these effects. (C) 1998 Elsevier Science B.V. All rights reserved.
