Improved Performance of UHPLC-MS Hyphenated Systems

被引：0
作者：
Gritti, Fabrice [1 ]
Meyyappan, Sornanathan [2 ]
Leveille, Wade [2 ]
Hill, Jason [2 ]
机构：
[1] Waters Corp, Instrument Core Res Fundamentals Dept, Milford, MA 01757 USA
[2] Waters Corp, Milford, MA USA
来源：
LCGC NORTH AMERICA | 2022年 / 40卷 / 07期
关键词：
LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; PRESSURE; COLUMNS; TEMPERATURE;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An ultrahigh- pressure liquid chromatography-mass spectrometry (UHPLC- MS) research prototype instrument was built to improve the resolution power and the usability of conventional LC-MS hyphenated instruments for routine analyses in pharmaceutical applications. The improved characteristics of this UHPLC-MS system include: 1) the dramatic reduction of post-column sample dispersion; 2) the adoption of vacuum jacketed columns (VJC) for the reduction of undesirable radial temperature gradients across the column diameter; and 3) the presence of a column outlet end nut heater to refocus the distorted peaks prior to analyte ionization. The benefits of each of these added features are analyzed with a rigorous approach from a peak broadening perspective. A 2x improvement in peak capacities recorded with this prototype UHPLC- MS system compared to a standard system (Acquity UHPLC I-class/Xevo TQ-S) is illustrated for the gradient separation of seven small pharmaceutical compounds using a 2.1 mm x 100 mm column packed with sub-2-mu m core-shell particles (1.6 mu m Acquity UHPLC Cortecs C18 column).
引用
收藏
页码：296 / 303
页数：8
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