Enzyme characteristics of recombinant poly(ADP-ribose) polymerases-1 of rat and human origin mirror the correlation between cellular poly(ADP-ribosyl)ation capacity and species-specific life span

被引：30

作者：

Beneke, Sascha ^{[1
]}

Scherr, Anna-Lena ^{[1
]}

Ponath, Viviane ^{[1
]}

Popp, Oliver ^{[1
]}

Buerkle, Alexander ^{[1
]}

机构：

[1] Univ Konstanz, Dept Biol, Mol Toxicol Grp, D-78457 Constance, Germany

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2010年 / 131卷 / 05期

关键词：

Poly(ADP-ribose)polymerase-1; Enzyme activity; Life span; Allele; ACTIVATION; EXPRESSION; CHROMATIN; POLYMORPHISMS; RECRUITMENT; REVEALS; PROTEIN; CANCER; REPAIR; LINES;

D O I：

10.1016/j.mad.2010.04.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Poly(ADP-ribosyl)ation is a posttranslational modification, which is involved in many cellular functions, including DNA repair and maintenance of genomic stability, and has also been implicated in cellular and organismal ageing. We have previously reported that maximum poly(ADP-ribosyl)ation capacity in mononuclear blood cells is correlated with mammalian life span. Here we show that the difference between a long-lived and a short-lived species tested (i.e. man and rat) is directly mirrored by the enzymatic parameters of recombinant poly(ADP-ribose) polymerase-1 (PARP-1), i.e. substrate affinity and reaction velocity. In addition, we have characterized two human PARP-1 alleles and assign their activity difference to their respective initial velocity and not substrate affinity. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：366 / 369

页数：4

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