Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate Alexandrium tamarense

被引：14

作者：

Cho, Yuko ^{[1
]}

Ogawa, Motoo ^{[2
]}

Hirota, Mayumi ^{[3
]}

Oshima, Yasukatsu ^{[2
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan

[2] Tohoku Univ, Grad Sch Life Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan

[3] Tohoku Univ, Fac Agr, Aoba Ku, Sendai, Miyagi 9818555, Japan

来源：

HARMFUL ALGAE | 2011年 / 10卷 / 03期

关键词：

Mitomycin C; Colchicine; Alexandrium tamarense; Paralytic shellfish toxins; PARALYTIC SHELLFISH; GYMNODINIUM-CATENATUM; SAXITOXIN; DINOPHYCEAE; TOXICITY; GROWTH; TEMPERATURE; BIVALVES; DYNAMICS; DIVISION;

D O I：

10.1016/j.hal.2010.10.003

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

Paralytic shellfish toxin (PST) production and cell proliferation in the dinoflagellate Alexandrium tamarense were investigated under the influence of two metabolic inhibitors (mitomycin C and colchicine) that have different mechanisms of action. Intracellular PST levels in cells treated with 2 mu M mitomycin C increased gradually, reaching a maximum of 176 fmol/cell (a 6-fold increase). High concentrations of colchicine prolonged G(1) phase in A. tamarense cells, even though colchicine arrests several other eukaryotic cell types in M phase. The cells in prolonged G(1) phase under the influence of colchicine were apparently unable to produce PST. Cell proliferation and toxin production recovered after removal of colchicine; in contrast, the effect of mitomycin C was irreversible. While the timing of toxin production within the cell cycle was not conclusively determined, A. tamarense cells in S phase were able to produce PST. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：235 / 244

页数：10

共 43 条

[11]

HARADA T, 1982, B JPN SOC SCI FISH, V48, P821

[12] S-adenosylmethionine synthetase genes from eleven marine dinoflagellates [J].

Harlow, Lucy D. ;

Koutoulis, Anthony ;

Hallegraeff, Gustaaf M. .

PHYCOLOGIA, 2007, 46 (01) :46-53

[13] First paralytic shellfish poison (PSP) infestation of bivalves due to toxic dinoflagellate Alexandrium tamiyavanichii, in the southeast coasts of the Seto Inland Sea, Japan [J].

Hashimoto, T ;

Matsuoka, S ;

Yoshimatsu, SA ;

Miki, K ;

Nishibori, N ;

Nishio, S ;

Noguchi, T .

JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN, 2002, 43 (01) :1-5

[14] Chlorpropham [isopropyl N-(3-chlorophenyl) carbamate] disrupts microtubule organization, cell division, and early development of sea urchin embryos [J].

Holy, J .

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A, 1998, 54 (04) :319-333

[15] MOLECULAR ASPECTS OF MECHANISM OF ACTION OF 5-FLUORODEOXYURIDINE [J].

KALMAN, TI .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1975, 255 (AUG8) :326-331

[16] Biosynthetic intermediate analysis and functional homology reveal a saxitoxin gene cluster in cyanobacteria [J].

Kellmann, Ralf ;

Mihali, Troco Kaan ;

Jeon, Young Jae ;

Pickford, Russell ;

Pomati, Francesco ;

Neilan, Brett A. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (13) :4044-4053

[17] Symbiodinium (Pyrrhophyta) genome sizes (DNA content) are smallest among dinoflagellates [J].

LaJeunesse, TC ;

Lambert, G ;

Andersen, RA ;

Coffroth, MA ;

Galbraith, DW .

JOURNAL OF PHYCOLOGY, 2005, 41 (04) :880-886

[18] Three functional luciferase domains in a single polypeptide chain [J].

Li, LM ;

Hong, R ;

Hastings, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (17) :8954-8958

[19] Salinity effect on growth and toxin production of four tropical Alexandrium species (Dinophyceae) [J].

Lim, PT ;

Ogata, T .

TOXICON, 2005, 45 (06) :699-710

[20] The dinoflagellate genus Alexandrium (Halim) in New Zealand coastal waters:: comparative morphology, toxicity and molecular genetics [J].

MacKenzie, L ;

de Salas, M ;

Adamson, J ;

Beuzenberg, V .

HARMFUL ALGAE, 2004, 3 (01) :71-92

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