Antihypertensive activity of 80% methanol seed extract of Calpurnia aurea (Ait.) Benth. subsp aurea (Fabaceae) is mediated through calcium antagonism induced vasodilation

Ethnopharmacological relevance: Calpurnia aurea (Alt.) Benth. subsp. aurea (CASA) (Fabaceae) seeds are used to treat hypertension in Ethiopian folklore medicine, particularly by Shinasha, Agew-awi and Amhara people in northwest Ethiopia. However, the claim has so far not been substantiated scientifically. Aim of the study: The study was conducted to evaluate the antihypertensive activity of 80% methanol extract of CASA in animal model of hypertension as well as its vasorelaxant effect and possible underlying mechanisms in isolated guinea pig aorta. Material and methods: Hypotensive and antihypertensive effect of CASA extract was determined in vivo through the intravenous (iv) route in normotensive and hypertensive anesthetized rats using 2-kidney-1 clip (2K1C) rat model. Ex vivo, guinea pig thoracic aortic rings were isolated and suspended in organ bath, and the vasodepressor effects as well as the mechanism of action of the extract were studied by means of isometric tension recording experiments. Results: The blood pressure fell dose-dependently and significantly in renal hypertensive and normotensive rats following i.v. administration, suggesting that the hydroalcoholic extract possesses hypotensive and antihypertensive effects. The extract also caused a dose-dependent relaxation of aorta pre contracted with KCl at a concentration of 5-250 mg/L, with a maximum relaxation of 92.1% achieved at 250 mg/L. The relaxation mechanism was found to be independent of the muscarinic receptors, histamine receptors, ATP dependent K+ channels, cyclooxygenase enzymes, cGMP/NO pathway and the endothelium system. The extract caused rightward shift of the Ca++ dose response curves, similar to that caused by verapamil, indicating that it produced vasorelaxation by inhibiting extracellular Ca2+ influx. Conclusions: The findings demonstrate that the plant is endowed with antihypertensive effect, providing evidence for its traditional use. The effect may be, at least in part, due to dilation of blood vessels through blockage of Ca2+ channels. (C) 2016 Elsevier Ireland Ltd. All rights reserved.