Uniaxial cyclic tensile stretch inhibits osteogenic and odontogenic differentiation of human dental pulp stem cells

被引：27

作者：

Cai, Xiaoxiao ^{[2
]}

Zhang, Yi ^{[3
]}

Yang, Xingmei ^{[2
]}

Grottkau, Brian E. ^{[1
,4
]}

Lin, Yunfeng ^{[1
,2
,4
]}

机构：

[1] MassGen Hosp Children, Dept Orthopaed Surg, Boston, MA USA

[2] Sichuan Univ, W China Coll Stomatol, State Key Lab Oral Dis, Chengdu 610064, Peoples R China

[3] Chongqing Med Univ, Coll Stomatol, Dept Orthodont, Chongqing, Peoples R China

[4] Harvard Univ, Sch Med, Pediat Orthopaed Lab Tissue Engn & Regenerat Med, Boston, MA USA

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2011年 / 5卷 / 05期

基金：

中国国家自然科学基金;

关键词：

human dental pulp stem cells; uniaxial cyclic tensile stretch; mechanical stress; odontogenesis; osteogenesis; SEQUENCE DETERMINATION; TOOTH MORPHOGENESIS; IN-VITRO; EXPRESSION; TISSUE; DENTINOGENESIS; PHOSPHOPROTEIN; GENE; MSCS; VIVO;

D O I：

10.1002/term.319

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

As the most important organs of occlusion, teeth are subjected to a variety of mechanical stresses. These stresses are transmitted into the dental pulp tissue and affect the dental pulp stem cells. In this study, human dental pulp stem cells were isolated from human impacted third molars and their multilineage differentiation abilities were tested. Human dental pulp stem cells were then exposed to cyclic tensile stretch. The results showed that the cyclic tensile stretch inhibited the expression of osteogenic marker genes and proteins such as BMP-2, OCN and ALP. Simultaneously, odontogenic marker genes and proteins such as DSPP, DSP and BSP were also inhibited by the mechanical stress. It was concluded that cyclic tensile stretch inhibits the osteogenic and odontogenic differentiation of dental pulp stem cells. Copyright (C) 2010 John Wiley & Sons, Ltd.

引用

页码：347 / 353

页数：7

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