Evaluation of the CRE and ESBL ELITe MGB® kits for the accurate detection of carbapenemase- or CTX-M-producing bacteria

被引:19
作者
Girlich, Delphine [1 ,2 ]
Bernabeu, Sandrine [1 ,2 ,3 ]
Fortineau, Nicolas [1 ,2 ,3 ,4 ]
Dortet, Laurent [1 ,2 ,3 ,4 ]
Naas, Thierry [1 ,2 ,3 ,4 ]
机构
[1] Univ Paris Saclay, Univ Paris Sud, Struct Dynam Funct & Express Broad Spectrumss Lac, Fac Med,LabEx Lermit, Le Kremlin Bicetre, France
[2] Univ Paris Sud, AP HP, Inst Pasteur, Evolut & Ecol Resistance Antibiot Unit, Paris, France
[3] Bicetre Hosp, AP HP, Bacteriol Hyg Unit, Le Kremlin Bicetre, France
[4] Associated French Natl Reference Ctr Antibiot Res, Le Kremlin Bicetre, France
关键词
Multiplex PCR; Real-time detection; Screening; Confirmation; Carbapenemase; CARBA NP TEST; RAPID DETECTION; RESISTANT-ENTEROBACTERIACEAE; PREVALENCE; ALGORITHM;
D O I
10.1016/j.diagmicrobio.2018.02.001
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
As carbapenemase-producing Enterobacteriaceae (CPE) and extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. The ELITe InGenius is a fully automated sample-to-result system designed for the extraction and detection by multiplex real-time polymerase chain reaction of carbapenemases KPC, NDM, VIM, IMP, and OXA-48-like variants and CTX-M group 1 and 9-producers from diverse sample matrices such as colonies, positive blood cultures, and rectal swabs. CRE and ESBL ELITe MGB (R) kits were evaluated on 153 cultured colonies of enterobacterial isolates with characterized beta-lactamase content, on 30 spiked blood cultures, and the CRE kit was also evaluated on 53 clinical rectal swabs collected prospectively during a 3-month period and 10 spiked rectal swabs. CRE ELITe MGB (R) kit's performances reached 100% sensitivity and 100% specificity, while for the ESBL ELITe kit, 100% sensitivity and 96.6% specificity were observed, with a sample to result of less than 3 h and a total percentage of agreement with expected results of 99.6% (255/256). (C) 2018 Elsevier Inc. All rights reserved.
引用
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页码:1 / 7
页数:7
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