Mechanisms of protein fluorophore formation and engineering

被引：51

作者：

Miyawaki, A ^{[1
]}

Nagai, T ^{[1
]}

Mizuno, H ^{[1
]}

机构：

[1] RIKEN, Brain Sci Inst, Adv Technol Dev Grp, Lab Cell Funct Dynam, Wako, Saitama 3510198, Japan

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2003年 / 7卷 / 05期

关键词：

D O I：

10.1016/S1367-5931(03)00097-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-like proteins from Anthozoa species contain light-absorbing chromophores within their protein sequences. Recent studies have made progress in obtaining bright variants of these proteins that develop chromophores quickly and efficiently, as well as novel fluorescent proteins that photoconvert (i.e. change color upon illumination at specific wavelengths). Further molecular characterization of the structure and maturation of these proteins is in progress, aimed at providing information for rational design of variants with desired fluorescence properties.

引用

页码：557 / 562

页数：6

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