Impaired angiotensin II AT1 receptor function and enhanced Na, K-ATPase affinity for sodium in proximal tubule of streptozotocin-treated diabetic rats

被引：4

作者：

Siddiqui, Athar H. ^{[1
]}

Hussain, Tahir ^{[1
]}

机构：

[1] Univ Houston, Dept Pharmacol & Pharmaceut Sci, Houston, TX 77204 USA

来源：

CLINICAL AND EXPERIMENTAL HYPERTENSION | 2007年 / 29卷 / 07期

关键词：

angiotensin receptors; sodium pump; kidney; natriuresis;

D O I：

10.1080/10641960701615659

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We determined angiotensin II (Ang II) AT, receptor function in terms of Na-K-ATPAse (NKA) stimulation in the proximal tubule (PTs) of streptozotocin-induced diabetic rats. Ang II (10 pM) stimulated NKA activity in PTs of control rats but not diabetic rats. The AT, receptor expression was similar, but the expression of G-proteins (G(i)alpha 2 and G(i)alpha 3) in the PTs was decreased in diabetic compared with control rats. Kinetic studies revealed an increase in NKA affinity, low K-0.5, for Na, with no changes in V.. of the enzyme in diabetic compared with control rats. Basal Ser-phosphorylation of NKA alpha 1-subunit was lower in diabetic compared with control rats. This data suggest that the higher basal NKA affinity for Na, possibly due to lower Ser-phosphorylaion of alpha 1-subunit and not the AT, receptor function, in the PTs may be responsible for increased renal No reabsorption associated with early stage of streptozotocin-induced diabetes.

引用

页码：435 / 444

页数：10

共 38 条

[1] Hypertension in obese Zucker rats - Role of angiotensin II and adrenergic activity [J].

AlonsoGalicia, M ;

Brands, MW ;

Zappe, DH ;

Hall, JE .

HYPERTENSION, 1996, 28 (06) :1047-1054

[2] Activation of dopamine D1-like receptor causes phosphorylation of α1-subunit of Na+,K+-ATPase in rat renal proximal tubules [J].

Asghar, M ;

Hussain, T ;

Lokhandwala, MF .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2001, 411 (1-2) :61-66

[3] Insulin treatment enhances AT1 receptor function in OK cells [J].

Banday, AA ;

Siddiqui, AH ;

Menezes, MM ;

Hussain, T .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2005, 288 (06) :F1213-F1219

[4] PROGRESSIVE INCREASES IN LUMINAL GLUCOSE STIMULATE PROXIMAL SODIUM-ABSORPTION IN NORMAL AND DIABETIC RATS [J].

BANK, N ;

AYNEDJIAN, HS .

JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (01) :309-316

[5] Increased renal angiotensin II AT1 receptor function in obese Zucker rat [J].

Becker, M ;

Umrani, D ;

Lokhandwala, MF ;

Hussain, T .

CLINICAL AND EXPERIMENTAL HYPERTENSION, 2003, 25 (01) :35-47

[6] Angiotensin-converting enzyme inhibitors versus AT1 receptor antagonist in cardiovascular and renal protection:: The case for AT1 receptor antagonist [J].

Berl, T .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2004, 15 (01) :S71-S76

[7] PHOSPHORYLATION OF THE CATALYTIC SUBUNIT OF NA+,K+-ATPASE INHIBITS THE ACTIVITY OF THE ENZYME [J].

BERTORELLO, AM ;

APERIA, A ;

WALAAS, SI ;

NAIRN, AC ;

GREENGARD, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11359-11362

[8] Tissue-specific changes in angiotensin II receptors in streptozotocin-diabetic rats [J].

Brown, L ;

Wall, D ;

Marchant, C ;

Sernia, C .

JOURNAL OF ENDOCRINOLOGY, 1997, 154 (02) :355-362

[9] REDUCED PROXIMAL TUBULE ANGIOTENSIN-II RECEPTOR EXPRESSION IN STREPTOZOTOCIN-INDUCED DIABETES-MELLITUS [J].

CHENG, HF ;

BURNS, KD ;

HARRIS, RC .

KIDNEY INTERNATIONAL, 1994, 46 (06) :1603-1610

[10]

de Gasparo M, 2000, PHARMACOL REV, V52, P415

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