Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter

被引：6

作者：

Maehara, Tomoko ^{[1
]}

Tomita, Shizuko ^{[1
]}

Takabatake, Koji ^{[2
]}

Kaneko, Satoshi ^{[1
]}

机构：

[1] Natl Food Res Inst, Food Biotechnol Div, Tsukuba, Ibaraki 3058642, Japan

[2] Toyama Prefectural Agr Forestry & Fisheries Res C, Forest Inst, Tateyama, Toyama 9301362, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2010年 / 74卷 / 12期

关键词：

Flammulina velutipes Fv-1; transformation; glyceraldehyde-3-phosphate dehydrogenase gene; promoter;

D O I：

10.1271/bbb.100556

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trpl) promoter.

引用

页码：2523 / 2525

页数：3

共 11 条

[1] Restriction enzyme-mediated DNA integration in Coprinus cinereus [J].

Granado, JD ;

KerteszChaloupkova, K ;

Aebi, M ;

Kues, U .

MOLECULAR AND GENERAL GENETICS, 1997, 256 (01) :28-36

[2] ISOLATION AND IDENTIFICATION OF YEAST MESSENGER RIBONUCLEIC-ACIDS CODING FOR ENOLASE, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, AND PHOSPHOGLYCERATE KINASE [J].

HOLLAND, MJ ;

HOLLAND, JP .

BIOCHEMISTRY, 1978, 17 (23) :4900-4907

[3] Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes [J].

Kuo, CY ;

Chou, SY ;

Huang, CT .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 65 (05) :593-599

[4] Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain [J].

Maehara, Tomoko ;

Yoshida, Makoto ;

Ito, Yasuhiro ;

Tomita, Shizuko ;

Takabatake, Koji ;

Ichinose, Hitomi ;

Kaneko, Satoshi .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2010, 74 (05) :1126-1128

[5] Properties of Ethanol Fermentation by Flammulina velutipes [J].

Mizuno, Ryoji ;

Ichinose, Hitomi ;

Maehara, Tomoko ;

Takabatake, Koji ;

Kaneko, Satoshi .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2009, 73 (10) :2240-2245

[6] Use of Whole Crop Sorghums as a Raw Material in Consolidated Bioprocessing Bioethanol Production Using Flammulina velutipes [J].

Mizuno, Ryoji ;

Ichinose, Hitomi ;

Honda, Mariko ;

Takabatake, Koji ;

Sotome, Itaru ;

Takai, Tomoyuki ;

Maehara, Tomoko ;

Okadome, Hiroshi ;

Isobe, Seiichiro ;

Gau, Mitsuru ;

Kaneko, Satoshi .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2009, 73 (07) :1671-1673

[7] POST-TRANSCRIPTIONAL REGULATION OF GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE GENE-EXPRESSION IN RAT-TISSUES [J].

PIECHACZYK, M ;

BLANCHARD, JM ;

MARTY, L ;

DANI, C ;

PANABIERES, F ;

ELSABOUTY, S ;

FORT, P ;

JEANTEUR, P .

NUCLEIC ACIDS RESEARCH, 1984, 12 (18) :6951-6963

[8] FUNCTIONAL ELEMENTS IN THE PROMOTER REGION OF THE ASPERGILLUS-NIDULANS GPDA GENE ENCODING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE [J].

PUNT, PJ ;

DINGEMANSE, MA ;

KUYVENHOVEN, A ;

SOEDE, RDM ;

POUWELS, PH ;

VANDENHONDEL, CAMJJ .

GENE, 1990, 93 (01) :101-109

[9]

Sambrook J., 2001, MOL CLONING, V3rd, pA8

[10] Consolidated bioprocessing for bioethanol production using Saccharomyces cereviside [J].

van Zyl, Willem H. ;

Lynd, Lee R. ;

den Haan, Riaan ;

McBride, John E. .

BIOFUELS, 2007, 108 :205-235

← 1 2 →