A portable 3D-printed biosensing device for rapid detection of genetically modified maize MON810

被引：9
作者：
Zhang, Qian [1 ]
Wang, Weiran [1 ]
Yang, Zhansen [1 ]
Wang, Xin [1 ]
Xu, Wentao [1 ,2 ]
Huang, Kunlun [1 ,2 ]
Luo, Yunbo [1 ,2 ]
He, Xiaoyun [1 ,2 ]
Cheng, Nan [1 ,2 ]
机构：
[1] China Agr Univ, Beijing Lab Food Qual & Safety, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
[2] Key Lab Safety Assessment Genetically Modified Or, Minist Agr, Beijing 100083, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2021年 / 349卷
关键词：
Genetically modified maize MON810; Event-specific RPA; Lateral flow strip; Rapid detection; 3D-printed biosensing device; TRANSGENIC-MAIZE; SAFETY; ASSAYS; LEVEL; FIELD; GENE; GMO;
D O I：
10.1016/j.snb.2021.130748
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Portable 3D-printed biosensing devices have high potential as analytical platforms due to their unique characteristics. In this study, an easy-to-use biosensing device was constructed based on an event-specific recombinase polymerase amplification-lateral flow strip (RPA-LFS) method for rapid detection of genetically modified maize, MON810. We first established a rapid method for genomic DNA extraction, which achieved rapid extraction in 5 min and does not require professional equipment. The extracted DNA was then used for event-specific RPA amplification and LFS detection in a self-designed 3D-printed device that realized the detection of genetically modified maize MON810 in less than 25 min. The whole process from raw material processing to detection was rapid and easy to use. The qualitative detection limit of the method established in this study was 0.1 wt%, which meets the requirements of most countries and regions in the world for the detection of genetically modified ingredients. The results indicated that the portable 3D-printed biosensing device was sensitive and reliable with actual samples and has the potential to be applied to the rapid screening of risk factors for food safety.
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页数：9
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