First report of pink root rot caused by Setophoma (Pyrenochaeta) terrestris on canola

被引：18

作者：

Yang, Yalong ^{[1
]}

Zuzak, Krista ^{[1
]}

Harding, Michael ^{[2
]}

Neilson, Eric ^{[3
]}

Feindel, David ^{[1
]}

Feng, Jie ^{[1
]}

机构：

[1] Alberta Agr & Forestry, Alberta Plant Hlth Lab, Edmonton, AB T5Y 6H3, Canada

[2] Alberta Agr & Forestry, Crop Diversificat Ctr South, Brooks, AB T1R 1E6, Canada

[3] Battle River Res Grp, Forestburg, AB T0B 1N0, Canada

来源：

CANADIAN JOURNAL OF PLANT PATHOLOGY | 2017年 / 39卷 / 03期

关键词：

DNA barcoding; elongation factor; internal transcribed spacer; pathogenicity; PCR; Pyrenochaeta; beta-tubulin; FUNGUS; DISEASE;

D O I：

10.1080/07060661.2017.1355849

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Diseased canola plants which failed to produce pods and were shorter than healthy plants in the same field were submitted to the Alberta Plant Health Laboratory in September 2016. The predominant symptom on infected plants was root rot, resembling a pink root rot. The objective of this study was to confirm the occurrence of canola pink root rot in Alberta. Two of the fungal strains isolated from symptomatic root samples were subjected to DNA barcoding that targeted three genomic regions, namely the internal transcribed spacer (ITS) region, the translation elongation factor-1 alpha gene (EF1) and the beta-tubulin gene. By similarity search (BlastN) using the resultant sequences as queries against GenBank accession sequences, both isolates were identified as Setophoma terrestris. Experiments were conducted to fulfil Koch's postulates to demonstrate that this fungal species is a pathogen of canola. After inoculation, the two strains could cause pink root rot on canola. The fungal strains were re-isolated from the inoculated canola and their identity confirmed as S. terrestris. This is the first report of pink root rot on canola and on any Brassica host in the world.

引用

页码：354 / 360

页数：7

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