Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

被引:0
|
作者
De Luca, Anna Chiara [1 ]
Mazilu, Michael [1 ]
Riches, Andrew [2 ]
Herrington, Simon [2 ]
Dholakia, Kishan [1 ]
机构
[1] Univ St Andrews North Haugh, SUPA, Sch Phys & Astron, St Andrews KY16 9SS, Fife, Scotland
[2] Univ St Andrews, Bute Med Sch, St Andrews KY16 9TS, Fife, Scotland
来源
ADVANCED BIOMEDICAL AND CLINICAL DIAGNOSTIC SYSTEMS VIII | 2010年 / 7555卷
基金
英国工程与自然科学研究理事会;
关键词
Raman spectroscopy; fluorescence-free; PCA; REJECTION;
D O I
10.1117/12.841686
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.
引用
收藏
页数:6
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