Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro

被引：14

作者：

Hu, Han ^{[1
,2
]}

Zheng, Nan ^{[1
]}

Gao, Haina ^{[2
]}

Dai, Wenting ^{[2
]}

Zhang, Yangdong ^{[2
]}

Li, Songli ^{[2
]}

Wang, Jiaqi ^{[2
]}

机构：

[1] Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Minist Agr,Milk Risk Assessment Lab, Beijing 100193, Peoples R China

[2] Chinese Acad Agr Sci, Inst Apicultural Res, Minist Agr, Key Lab Pollinating Biol, Beijing 100193, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2016年 / 40卷 / 08期

关键词：

bovine mammary epithelial cell; differentiation; immortalization; stem cell; MAC-T; GENE-EXPRESSION; LINE; ESTABLISHMENT; P63; MORPHOLOGY; LACTATION; NETWORKS; PROTEINS;

D O I：

10.1002/cbin.10624

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The bovine mammary epithelial cell is a secretory cell, and its cell number and secretory activity determine milk production. In this study, we immortalized a bovine mammary epithelial cell line by SV40 large T antigen gene using a retrovirus based on Chinese Holstein primary mammary epithelial cells (CMEC) cultured in vitro. An immortalized bovine mammary epithelial cell line surpassed the 50-passage mark and was designated the CMEC-H. The immortalized mammary epithelial cells grew in close contact with each other and exhibited the typical cobblestone morphology characteristic with obvious boundaries. The telomerase expression of CMEC-H has consistently demonstrated the presence of telomerase activity as an immortalized cell line, but the cell line never induced tumor formation in nude mice. CMEC-H expressed epithelial (cytokeratins CK7, CK8, CK18, and CK19), mesenchymal (vimentin), and stem/progenitor (CD44 and p63) cell markers. The induced expression of milk proteins, (S1)-casein, -casein, -casein, and butyrophilin, indicated that CMEC-H maintained the synthesis function of the mammary epithelial cells. The established immortalized bovine mammary epithelial cell line CMEC-H is capable of self-renewal and differentiation and can serve as a valuable reagent for studying the physiological mechanism of the mammary gland.

引用

页码：861 / 872

页数：12