Enzymes of SPZ7 phage: Isolation and properties

被引：4

作者：

Levashov, P. A. ^{[1
]}

Popov, D. V. ^{[2
]}

Popova, V. M. ^{[2
]}

Zhilenkov, E. L. ^{[2
]}

Morozova, O. A. ^{[3
]}

Belogurova, N. G. ^{[1
]}

Sedov, S. A. ^{[1
]}

Dyatlov, I. A. ^{[2
]}

Klyachko, N. L. ^{[1
]}

Levashov, A. V. ^{[1
]}

机构：

[1] Lomonosov Moscow State Univ, Fac Chem, Moscow 119991, Russia

[2] State Res Ctr Appl Microbiol & Biotechnol, Obolensk 142279, Moscow Region, Russia

[3] Sechenov Moscow Med Acad, Ctr Clin, Bacteriol Lab, Moscow 119021, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2010年 / 75卷 / 09期

关键词：

bacteriophage enzyme; lysis of bacteria; bacteriolytic activity; SALMONELLA-ENTERITIDIS;

D O I：

10.1134/S0006297910090105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacteriophage enzyme preparations exolysin and endolysin were studied. Exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated Salmonella enteritidis cells. A new method for purification of these enzymes was developed, and their molecular masses were determined. The main catalytic properties of the studied enzymes (pH optimum and specificity to bacterial substrates) were found to be similar. Both enzymes lyse Escherichia coli cells like chicken egg lysozyme, but more efficiently lyse S. enteritidis cells and cannot lyse Micrococcus luteus, a good substrate for chicken egg lysozyme. Similar properties of exolysin and endolysin suggest that these enzymes are structurally similar or even identical.

引用

页码：1160 / 1164

页数：5

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