Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine

被引：13
|
作者：
Hossain, Mohammad M. [1 ,2 ]
Wilson, William C. [2 ]
Faburay, Bonto [1 ]
Richt, Jurgen [1 ]
McVey, David S. [2 ]
Rowland, Raymond R. [1 ]
机构：
[1] Kansas State Univ, Coll Vet Med, CEEZAD, Dept Diagnost Med Pathobiol, 1800 Denison Ave,N-211 Mosier Hall, Manhattan, KS 66506 USA
[2] ARS, USDA, ABADRU, Ctr Grain & Anim Hlth Res, Manhattan, KS USA
来源：
VECTOR-BORNE AND ZOONOTIC DISEASES | 2016年 / 16卷 / 08期
关键词：
Fluorescence microsphere immunoassay; IgM antibody response; Recombinant RVFV proteins; Rift Valley fever virus; DOMESTIC RUMINANTS; CLIMATE-CHANGE; VACCINATED ANIMALS; POTENTIAL VECTORS; INDIRECT ELISA; CAPTURE ELISA; UNITED-STATES; SAUDI-ARABIA; N-PROTEIN; NSM GENES;
D O I：
10.1089/vbz.2014.1721
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A multiplex fluorescence microsphere immunoassay (FMIA) was used to detect bovine and ovine IgM and IgG antibodies to several Rift Valley fever virus (RVFV) proteins, including the major surface glycoprotein, Gn; the nonstructural proteins, NSs and NSm; and the nucleoprotein, N. Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural proteins, which can be applied to future development and validation of diagnostic tests that can be used to differentiate vaccinated from infected animals.
引用
收藏
页码：550 / 557
页数：8
相关论文
共 50 条
  • [1] Generation and Characterization of Monoclonal Antibodies Against Rift Valley Fever Virus Nucleoprotein
    Fafetine, J. M.
    Domingos, A.
    Antunes, S.
    Esteves, A.
    Paweska, J. T.
    Coetzer, J. A. W.
    Rutten, V. P. M. G.
    Neves, L.
    TRANSBOUNDARY AND EMERGING DISEASES, 2013, 60 : 24 - 30
  • [2] Validation of an IgM antibody capture ELISA based on a recombinant nucleoprotein for identification of domestic ruminants infected with Rift Valley fever virus
    Williams, Roy
    Ellis, Charlotte Elizabeth
    Smith, Shirley Jacqueline
    Potgieter, Christiaan Abraham
    Wallace, David
    Mareledwane, Vuyokazi Epipodia
    Majiwa, Phelix Antipas Ochola
    JOURNAL OF VIROLOGICAL METHODS, 2011, 177 (02) : 140 - 146
  • [3] Validation of an ELISA for the concurrent detection of total antibodies (IgM and IgG) to Rift Valley fever virus
    Ellis, Charlotte E.
    Mareledwane, Vuyokazi E.
    Williams, Roy
    Wallace, David B.
    Majiwa, Phelix A. O.
    ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH, 2014, 81 (01)
  • [4] Rift Valley Fever Virus Structural and Nonstructural Proteins: Recombinant Protein Expression and Immunoreactivity Against Antisera from Sheep
    Faburay, Bonto
    Wilson, William
    McVey, D. Scott
    Drolet, Barbara S.
    Weingartl, Hana
    Madden, Daniel
    Young, Alan
    Ma, Wenjun
    Richt, Juergen A.
    VECTOR-BORNE AND ZOONOTIC DISEASES, 2013, 13 (09) : 619 - 629
  • [5] Antigen-capture ELISA for the detection of Rift Valley fever virus nucleoprotein using new monoclonal antibodies
    Fukushi, Shuetsu
    Nakauchi, Mina
    Mizutani, Tetsuya
    Saijo, Masayuki
    Kurane, Ichiro
    Morikawa, Shigeru
    JOURNAL OF VIROLOGICAL METHODS, 2012, 180 (1-2) : 68 - 74
  • [6] A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants
    Jaeckel, S.
    Eiden, M.
    Balkema-Buschmann, A.
    Ziller, M.
    van Vuren, P. Jansen
    Paweska, J. T.
    Groschup, M. H.
    RESEARCH IN VETERINARY SCIENCE, 2013, 95 (02) : 725 - 730
  • [7] Validation of IgG-sandwich and IgM-capture ELISA for the detection of antibody to Rift Valley fever virus in humans
    Paweska, JT
    Burt, FJ
    Swanepoel, R
    JOURNAL OF VIROLOGICAL METHODS, 2005, 124 (1-2) : 173 - 181
  • [8] Bead-based suspension array for simultaneous detection of antibodies against the Rift Valley fever virus nucleocapsid and Gn glycoprotein
    van der Wal, Fimme J.
    Achterberg, Rene P.
    de Boer, S. Matthijn
    Boshra, Hani
    Brun, Alejandro
    Maassen, Catharina B. M.
    Kortekaas, Jeroen
    JOURNAL OF VIROLOGICAL METHODS, 2012, 183 (02) : 99 - 105
  • [9] Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans
    Sobarzo, A.
    Paweska, J. T.
    Herrmann, S.
    Amir, T.
    Marks, R. S.
    Lobel, L.
    JOURNAL OF VIROLOGICAL METHODS, 2007, 146 (1-2) : 327 - 334
  • [10] IgG-sandwich and IgM-capture enzyme-linked immunosorbent assay for the detection of antibody to Rift Valley fever virus in domestic ruminants
    Paweska, JT
    Burt, FJ
    Anthony, F
    Smith, SJ
    Grobbelaar, AA
    Croft, JE
    Ksiazek, TG
    Swanepoel, R
    JOURNAL OF VIROLOGICAL METHODS, 2003, 113 (02) : 103 - 112
12345