Densitometric HPTLC Method for Analysis of Oleanolic Acid in Achyranthes aspera L.

被引：4
作者：
Mehta, Falgun A. [1 ]
Patel, Bharat G. [2 ]
Pandya, Saurabh S. [3 ]
Ahir, Keyur B. [1 ]
机构：
[1] Indukaka Ipcowala Coll Pharm, New Vallabh Vidyanagar 388121, Gujarat, India
[2] AR Coll Pharm, Vallabh Vidyanagar 388120, Gujarat, India
[3] Babariya Coll Pharm, Vadodara 391240, Gujarat, India
来源：
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2010年 / 23卷 / 04期
关键词：
High-performance thin-layer chromatography (HPTLC); Oleanolic acid; Triterpenoid saponins; Achyranthes aspera; CHROMATOGRAPHIC DETERMINATION; PLANT SAPONINS;
D O I：
10.1556/JPC.23.2010.4.10
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A densitometric HPTLC method has been established for analysis in alcohol extracts of the seeds and leaves of Achyranthes aspera L. The extracts were separated on aluminum foil-backed silica gel 60 F(254) plates. Detection was by measurement of absorbance at 490 nm, in absorbance/reflectance mode, after derivatization with 10% H(2)SO(4) in 95.5% (v/v) ethanol. The R(F) of oleanolic acid was 0.22. The oleanolic acid content of both extracts was compared statistically and the amount in the seed extract found to be higher (0.970%) than that in the leaf extract (0.666%).
引用
收藏
页码：289 / 292
页数：4
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