Proteomics analysis of human cerebrospinal fluid

被引:91
|
作者
Yuan, XL
Desiderio, DM
机构
[1] Univ Tennessee, Ctr Hlth Sci, Charles B Stout Neurosci Mass Spectrometry Lab, Memphis, TN 38163 USA
[2] Univ Tennessee, Ctr Hlth Sci, Dept Neurol, Memphis, TN 38163 USA
[3] Univ Tennessee, Ctr Hlth Sci, Dept Mol Sci, Memphis, TN 38163 USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 815卷 / 1-2期
关键词
reviews; proteomics; cerebrospinal fluid;
D O I
10.1016/j.jchromb.2004.06.044
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cerebrospinal fluid (CSF) is secreted from several different central nervous system (CNS) structures, and any changes in the CSF composition will accurately reflect pathological processes. Proteomics offers a comprehensive bird's eye view to analyze CSF proteins at a systems level. This paper reviews the variety of analytical methods that have been used for proteomics analysis of CSF, including sample preparation, two-dimensional liquid and gel electrophoresis, mass spectrometry, bioinformatics, and non-gel methods. The differentially expressed CSF proteins that have been identified by proteomics methods are discussed. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:179 / 189
页数:11
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