Diversity of aquaporin mRNA expressed by rat and human retinas

被引：56
作者：
Tenckhoff, S
Hollborn, M
Kohen, L
Wolf, S
Wiedemann, P
Bringmann, A
机构：
[1] Univ Leipzig, Fac Med, Dept Ophthalmol, D-04103 Leipzig, Germany
[2] Univ Leipzig, Fac Med, Eye Clin, D-04103 Leipzig, Germany
来源：
NEUROREPORT | 2005年 / 16卷 / 01期
关键词：
aquaporins; glia; Muller cell; potassium channels; retina; RT-PCR;
D O I：
10.1097/00001756-200501190-00013
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Muller glial cells of the sensory retina mediate K+ and water fluxes that are facilitated by aquaporin-4 (AQP4) water channels and by Kir4.1-K+ channels. However, it is not known which subtypes of aquaporins are expressed in the mammalian retina. Using RTPCR, we found that both human and rat retinas express mRNA for a diversity of water channel proteins. The human retina expresses mRNAs for AQP0 to AQP12 proteins. Using real-time PCR, we found that the mRNAs for AQP4 and Kir4.1 are downregulated in retinas that were obtained from patients with proliferative retinopathy compared with post-mortem controls. The data suggest that the development of proliferative gliosis is accompanied by disturbed transglial water and ion movements. (C) 2005 Lippincott Williams Wilkins.
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收藏
页码：53 / 56
页数：4
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