Occurrence and molecular identification of anisakid nematodes isolated from Pacific cod (Gadus macrocephalus) caught off Korea

被引：5
作者：
Jeon, Chan-Hyeok [1 ,2 ]
Setyobudi, Eko [1 ,3 ]
Kim, Jeong-Ho [1 ]
机构：
[1] Gangneung Wonju Natl Univ, Dept Marine Biosci, Kangnung 210702, South Korea
[2] Gangneung Wonju Natl Univ, Inst East Coast Life Sci, Kangnung 210702, South Korea
[3] Gadjah Mada Univ, Dept Fisheries, Fac Agr, JL Flora, Bulaksumur 55281, Yogyakarta, Indonesia
来源：
FOOD CONTROL | 2016年 / 69卷
关键词：
Anisakis pegreffii; Hysterothylacium aduncum; Pacific cod; Gadus macrochepalus; Anisakid nematodes; ATLANTIC COD; HYSTEROTHYLACIUM-ADUNCUM; RIBOSOMAL DNA; MARINE FISHES; YELLOW SEA; MORHUA L; LARVAE; ASCARIDOIDEA; SIMPLEX; INFECTION;
D O I：
10.1016/j.foodcont.2016.04.031
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Anisakids nematodes from Pacific cod (Gadus macrocephalus) in Korea were investigated and their molecular identification was conducted, to assess the epidemiological role of Pacific cod in human anisakidosis in Korea. Totally 238 Pacific cod were caught from 5 different areas around Korean peninsula. Fish were dissected and carefully examined for collecting nematodes. PCR-RFLP and the subsequent sequencing were conducted for molecular identification of those nematodes. A high prevalence of infection (193/238, 81.1%) in Pacific cod was observed, and 1694 nematodes were-collected. 79.1%.(1340/1694) of the nematodes were found freely in the body cavity of Pacific cod, and the rest of them (20.9%, 354/1694) were in the digestive tract or attached to other organs. PCR-RFLP analysis using HinfI and Rsal restriction enzymes revealed 3 different banding patterns corresponding to Anisakis pegreffii, Hyster-othylacium aduncum and hybrid genotype (Anisakis simplex x A. pegreffii), respectively. Of 1694 nematodes, 1280 (75.6%) were identified as A. pegreffii and 406 (24.0%) were H. aduncum. A. pegreffii occupied 84.0% (1125/1340) of the nematodes in the body cavity and 40.0% (132/330) of them in the digestive tract, but no nematodes were found in Pacific cod muscles. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：100 / 107
页数：8
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