A method for simple and accurate identification of the multiple sclerosis associated allele HLA-DRB1*1501 in neuroscience research laboratories

被引：4

作者：

Cisneros, E. ^{[1
]}

Moraru, M. ^{[1
]}

de Pablo, R. ^{[1
]}

Vilches, C. ^{[1
]}

机构：

[1] Hosp Univ Puerta Hierro, Inmunogenet HLA, Majadahonda 28220, Spain

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2010年 / 225卷 / 1-2期

关键词：

DNA sequence analysis; Genetic predisposition to disease; Genetic screening; HLA-DR2; antigen; Human; Multiple sclerosis; Polymerase-chain reaction; POLYMERASE-CHAIN-REACTION; PRIMERS PCR-SSP; CLASS-II GENES; HLA-DR; MHC HAPLOTYPES; SUSCEPTIBILITY; HLA-DRB1; LOCUS; RISK; REGION;

D O I：

10.1016/j.jneuroim.2010.03.019

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Research on multiple sclerosis (MS) frequently requires typing for allele HLA-DRB1*1501, which the complexities of the HLA system can restrict to specialised histocompatibility laboratories. To overcome this limitation, we have implemented a simple, robust and highly specific method for DRB1*1501 detection. One single-tube polymerase-chain reaction (PCR) per DNA sample allows for detecting DR2 individuals. The spare PCR products of these are then sequenced to identify allele DRB1*1501 by comparison with the official, publicly accessible HLA database. This approach, much simpler than previously available methods, should facilitate research on MS by making accurate identification of DRB1*1501 accessible to neuroscience laboratories. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：143 / 148

页数：6

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