Simultaneous two-wavelength transmission quantitative phase microscopy with a color camera

被引:63
作者
Rinehart, Matthew T. [1 ]
Shaked, Natan T. [1 ]
Jenness, Nathan J. [2 ]
Clark, Robert L. [2 ]
Wax, Adam [1 ]
机构
[1] Duke Univ, Fitzpatrick Inst Photon, Dept Biomed Engn, Durham, NC 27708 USA
[2] Duke Univ, Dept Mech Engn & Mat Sci, Durham, NC 27708 USA
基金
美国国家科学基金会;
关键词
MULTIWAVELENGTH DIGITAL HOLOGRAPHY; SHIFTING INTERFEROMETRY;
D O I
10.1364/OL.35.002612
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We present a quantitative phase microscopy method that uses a Bayer mosaic color camera to simultaneously acquire off-axis interferograms in transmission mode at two distinct wavelengths. Wrapped phase information is processed using a two-wavelength algorithm to extend the range of the optical path delay measurements that can be detected using a single temporal acquisition. We experimentally demonstrate this technique by acquiring the phase profiles of optically clear microstructures without 2 pi ambiguities. In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified. (C) 2010 Optical Society of America
引用
收藏
页码:2612 / 2614
页数:3
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