Assembly of Selective Biomimetic Surface on an Electrode Surface: A Design of Nano-Bio Interface for Biosensing

被引：30

作者：

Gao, Tao ^{[1
]}

Liu, Fengzhen ^{[2
]}

Yang, Dawei ^{[1
]}

Yu, Yue ^{[3
]}

Wang, Zhaoxia ^{[2
]}

Li, Genxi ^{[1
,4
]}

机构：

[1] Nanjing Univ, Dept Biochem, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Affiliated Hosp 2, Dept Oncol, Nanjing 210011, Jiangsu, Peoples R China

[3] Nanjing Univ, Affiliated Hosp, Nanjing Drum Tower Hosp, Dept Hepatobiliary Surg, Nanjing 210008, Jiangsu, Peoples R China

[4] Shanghai Univ, Sch Life Sci, Lab Biosensing Technol, Shanghai 200444, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 11期

基金：

中国国家自然科学基金;

关键词：

PROTEIN-NANOPARTICLE INTERACTIONS; HORSERADISH-PEROXIDASE; TRANSFER REACTIVITY; GOLD NANOPARTICLE; CYTOCHROME-C; DNA; GRAPHENE; NANOMATERIALS; OXIDE; CELLS;

D O I：

10.1021/acs.analchem.5b00816

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In nature, cellular molecule sensing is usually achieved at the environment/membrane interface. In the meantime, rapid growth of nanotechnology is increasingly pushing engineered nanomaterials to interact with biological surfaces. Herein, inspired by trans-membrane signal transduction, a nano-bio interface has been constructed in this work for biosensing application. The interface is formed between a selective biomembrane mimetic surface (SBMMS) and a function-oriented 2D nanohybrid. Based on the design, target recognition can be performed in a biologically favorable environment, and the nanobio interaction can be transduced into amplified electrochemical readouts. Furthermore, this sensing platform can be used to analyze various kinds of targets, including proteins, nucleic acids, and small molecules, just by changing the biorecognition element. Low detection limits and wide detection ranges can also be obtained. So, this nano-bio interface may provide a new platform for bioanalytical research in the future.

引用

页码：5683 / 5689

页数：7

共 58 条

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