Downregulation of DLEC1 in Sinonasal Inverted Papilloma and Squamous Cell Carcinoma

被引：8
作者：
Chang, Po-Hung [1 ,2 ]
Huang, Chi-Che [1 ]
Lee, Ta-Jen [2 ]
Lee, Yun-Shien [3 ]
Tsai, Chi-Neu [1 ]
机构：
[1] Chang Gung Univ, Grad Inst Clin Med Sci, Tao Yuan 333, Taiwan
[2] Chang Gung Mem Hosp, Dept Otolaryngol Head & Neck Surg, Tao Yuan, Taiwan
[3] Ming Chuan Univ, Dept Biotechnol, Tao Yuan, Taiwan
来源：
JOURNAL OF OTOLARYNGOLOGY-HEAD & NECK SURGERY | 2012年 / 41卷 / 02期
关键词：
DLEC1; DNA methylation; inverted papilloma; sinonasal; squamous cell carcinoma; TUMOR-SUPPRESSOR GENE; NASAL CAVITY; DESMOGLEIN; 3; METHYLATION; HEAD; CANCER; MALIGNANCIES; PATHOGENESIS;
D O I：
10.2310/7070.2011.110202
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
Objective: Inverted papilloma is associated with sinonasal squamous cell carcinoma. This study aimed to investigate the epigenetic regulation of the tumour suppressor gene DLEC1 in inverted papilloma and sinonasal squamous cell carcinoma. Methods: DLEC1 gene expression was investigated by quantitative real-time polymerase chain reaction (QRT-PCR) in normal mucosa, inverted papilloma, and squamous cell carcinoma tissues. Methylation-specific PCR and subsequent autosequencing were also used to examine the methylation status of DLEC1 promoter and the involved mechanism. DLEC1 expression in a large validation set was evaluated by immunohistochemistry (n = 25 in each group). Results: DLEC1 was downregulated in inverted papilloma and squamous cell carcinoma tissues compared to normal mucosa (p < .01 in QRT-PCR and immunohistochemistry), with squamous cell carcinoma more repressed (p < .05 in QRT-PCR and immunohistochemistry) than inverted papilloma tissues. DLEC1 promoter hypermethylation was found in squamous cell carcinoma tissues. Conclusion: Repression of DLEC1 in squamous cell carcinoma tissues is associated with promoter hypermethylation. DLEC1 is downregulated in sinonasal squamous cell carcinoma and inverted papilloma and has a distinct mechanism.
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页码：94 / 101
页数：8
相关论文
共 23 条
[1]   SINONASAL PAPILLOMAS - A REPORT OF 82 CASES IN COPENHAGEN COUNTY, INCLUDING A LONGITUDINAL EPIDEMIOLOGIC AND CLINICAL-STUDY [J].
BUCHWALD, C ;
FRANZMANN, MB ;
TOS, M .
LARYNGOSCOPE, 1995, 105 (01) :72-79
[2]  
Califano J, 1996, CANCER RES, V56, P2488
[3]   Inverted sinonasal papilloma - A molecular genetic appraisal of its putative status as a precursor to squamous cell carcinoma [J].
Califano, J ;
Koch, W ;
Sidransky, D ;
Westra, WH .
AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (01) :333-337
[4]   Transcriptional repression of DLEC1 associates with the depth of tumor invasion in oral squamous cell carcinoma [J].
Chan, Wei-Hsuan ;
Chang, Kai-Ping ;
Yang, Shih-Wei ;
Yao, Tsung-Chieh ;
Ko, Tzu-Yin ;
Lee, Yun-Shien ;
Tsai, Chia-Lung ;
Tsai, Chi-Neu .
ORAL ONCOLOGY, 2010, 46 (12) :874-879
[5]   Expression of epidermal growth factor receptor in the inverted papilloma and squamous cell carcinoma of nasal cavity [J].
Chao, Jiung-Chih ;
Fang, Sheen-Yie .
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2008, 265 (08) :917-922
[6]   Methylation of multiple genes as diagnostic and therapeutic markers in primary head and neck squamous cell carcinoma [J].
Chen, Kangmei ;
Sawhney, Raja ;
Khan, Mumtaz ;
Benninger, Michael S. ;
Hou, Zizheng ;
Sethi, Seema ;
Stephen, Josena K. ;
Worsham, Maria J. .
ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY, 2007, 133 (11) :1131-1138
[7]  
Daigo Y, 1999, CANCER RES, V59, P1966
[8]   Malignancies of the nasal cavity and paranasal sinuses: Long-term outcome with conventional or three-dimensional conformal radiotherapy [J].
Dirix, Piet ;
Nuyts, Sandra ;
Geussens, Yasmyne ;
Jorissen, Mark ;
Vander Poorten, Vincent ;
Fossion, Eric ;
Hermans, Robert ;
Van den Bogaert, Walter .
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2007, 69 (04) :1042-1050
[9]   Desmoglein 3 as a prognostic factor in lung cancer [J].
Fukuoka, Junya ;
Dracheva, Tatiana ;
Shih, Joanna H. ;
Hewitt, Stephen M. ;
Fujii, Takashi ;
Kishor, Aparna ;
Mann, Felecia ;
Shilo, Konstantin ;
Franks, Teri J. ;
Travis, William D. ;
Jen, Jin .
HUMAN PATHOLOGY, 2007, 38 (02) :276-283
[10]   Molecular pathogenesis of head and neck squamous cell carcinoma [J].
Hardisson, D .
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 2003, 260 (09) :502-508
123