Paramagnetic oligonucleotides: Contrast agents for magnetic resonance imaging with proton relaxation enhancement effects

被引：13
作者：
Hines, JV [1 ]
Ammar, GM
Buss, J
Schmalbrock, P
机构：
[1] Ohio State Univ, Coll Pharm, Div Med Chem & Pharmacognosy, Columbus, OH 43210 USA
[2] Ohio State Univ, Coll Med, Dept Radiol, Columbus, OH 43210 USA
来源：
BIOCONJUGATE CHEMISTRY | 1999年 / 10卷 / 02期
关键词：
D O I：
10.1021/bc980103v
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An antisense paramagnetic oligonucleotide analogue targeted to a model macromolecular receptor (5S rRNA) was prepared. The paramagnetic agent's relaxivity (dependence of the relaxation rate on paramagnetic agent concentration) in the presence and absence of the macromolecular receptor was measured at 1.5 and 6.3 T. The relaxivity of the targeted agent increased specifically in the presence of the macromolecular receptor (16% at 6.3 T and 15% at 1.5 T). This effect was specific for a paramagnetic oligonucleotide targeted to the receptor and was larger than the relaxivity enhancement due simply to receptor-induced viscosity differences. Maximizing this relaxivity enhancement of tumor targeted paramagnetic oligonucleotides will aid in contrast agent development for magnetic resonance imaging.
引用
收藏
页码：155 / 158
页数：4
相关论文
共 25 条
  • [1] SYNTHESIS OF 1-(PARA-ISOTHIOCYANATOBENZYL) DERIVATIVES OF DTPA AND EDTA - ANTIBODY LABELING AND TUMOR-IMAGING STUDIES
    BRECHBIEL, MW
    GANSOW, OA
    ATCHER, RW
    SCHLOM, J
    ESTEBAN, J
    SIMPSON, DE
    COLCHER, D
    [J]. INORGANIC CHEMISTRY, 1986, 25 (16) : 2772 - 2781
  • [2] CANTOR CR, 1980, BIOPHYSICAL CHEM, V2, P461
  • [3] In vivo imaging of inflammation using an aptamer inhibitor of human neutrophil elastase
    Charlton, J
    Sennello, J
    Smith, D
    [J]. CHEMISTRY & BIOLOGY, 1997, 4 (11): : 809 - 816
  • [4] DEROME AE, 1987, MODERN NMR TECHNIQUE, P86
  • [5] DEWANJEE MK, 1994, J NUCL MED, V35, P1054
  • [6] Water diffusion and exchange as they influence contrast enhancement
    Donahue, KM
    Weisskoff, RM
    Burstein, D
    [J]. JMRI-JOURNAL OF MAGNETIC RESONANCE IMAGING, 1997, 7 (01): : 102 - 110
  • [7] GORINGER HU, 1988, METHOD ENZYMOL, V164, P721
  • [8] GREENFIELD GB, 1993, SKELETAL RADIOL, V22, P77
  • [9] Minor groove hydration of DNA in solution:: Dependence on base composition and sequence
    Jóhannesson, H
    Halle, B
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (28) : 6859 - 6870
  • [10] KAZUNARI M, 1997, JPN J BIOCH, V122, P387
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