Determination of L-Lactic Acid Content in Foods by Enzyme-Based Amperometric Bioreactor

被引：7
|
作者：
Bori, Zsuzsanna [2 ]
Csiffary, Gabor [1 ]
Virag, Diana [2 ]
Toth-Markus, Marianna [2 ]
Kiss, Attila [1 ]
Adanyi, Nora [2 ]
机构：
[1] Eszterhazy Karoly Coll, EGERFOOD Reg Knowledge Ctr, H-3300 Eger, Hungary
[2] Cent Food Res Inst, H-1022 Budapest, Hungary
来源：
ELECTROANALYSIS | 2012年 / 24卷 / 01期
关键词：
Enzymes; Biosensors; Thin-layer enzyme cell; Lactate oxidase; Aerococcus viridans; FLOW-INJECTION ANALYSIS; L-LACTATE; MILK-PROTEINS; REDOX ENZYMES; BIOSENSOR; POLY(ALLYLAMINE); MICRODIALYSIS; CONFIGURATION; ELECTRODE; BEHAVIOR;
D O I：
10.1002/elan.201100409
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A thermostable lactate oxidase (from Aerococcus viridans) based bioreactor was elaborated for L-lactate determination in dairy products. The enzyme was immobilised in a thin-layer enzyme cell connected into a flow injection system with an amperometric detector. The linear measuring range was 0.11 mmol/L L-lactate (R2=0.99; linear regression equation: y=81.2x+3.3). The detection limit was 0.05 mmol/L. 40 Samples were analyzed per hour. Considering reproducibility: the signals measured were very stable for over 800 injections, after this period the activity of the cell decreased slowly. The method was used for the control of dairy products and for monitoring milk freshness.
引用
收藏
页码：158 / 164
页数：7
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