Micropropagation of the bromeliad Guzmania 'Hilda' via organogenesis and the effect of α-naphthaleneacetic acid on plantlet elongation

被引：7

作者：

Huang, Ping-Lung ^{[2
,3
]}

Liu, Zin-Huang ^{[2
]}

Chang, Min-Lang ^{[2
,4
]}

Liao, Li-Jen ^{[1
]}

机构：

[1] Natl Kaohsiung Normal Univ, Dept Biotechnol, Kaohsiung 824, Taiwan

[2] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 804, Taiwan

[3] Kaohsiung Dist Agr Res & Extens Stn, Pingtung 908, Taiwan

[4] Inst Livestock Res, Branch Heng Chun, Pingtung 946, Taiwan

来源：

SCIENTIA HORTICULTURAE | 2011年 / 130卷 / 04期

关键词：

Bromeliad; Floral organ; Organogenesis; Micropropagation; Plantlet elongation; IN-VITRO-PROPAGATION; ABSCISIC-ACID; SHOOT-BUDS; AUXIN; REGENERATION; CELLS; HYPOCOTYL; SEEDLINGS; ABA;

D O I：

10.1016/j.scienta.2011.08.035

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

This study established a highly effective micropropagation system to obtain good plantlet proliferation from floral organs via callus induction and bud differentiation in Guzmania 'Hilda' bromeliad. The best frequencies of organogenic callus formation (20% in petal and 35% in ovary explants) were obtained on media containing a combination of 1.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) with 1.0 mgl(-1) alpha-naphthaleneacetic acid (NAA) and 1.5 mgl(-1) 2,4-D with 0.5 mgl(-1) NAA, respectively. Organogenic calli were cultured on medium with 1.0 mgl(-1) NAA and 0.5 mgl(-1) 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea (TDZ) induce the differentiation and regeneration of adventitious buds into plantlets. When the plantlets were cultured in a medium with optimum NAA concentration (0.5-1.0 mgl(-1)) significant improvement in regeneration and elongation was achieved within one month. This overcame the difficulty of delayed elongation in Guzmania plantlets. More than 99% of the regenerated and acclimatized plantlets developed to the flowering stage. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：894 / 898

页数：5

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