Asymmetrical flow field-flow fractionation technique for separation and characterization of biopolymers and bioparticles

被引：123

作者：

Yohannes, G. ^{[1
]}

Jussila, M. ^{[1
]}

Hartonen, K. ^{[1
]}

Riekkola, M. -L. ^{[1
]}

机构：

[1] Univ Helsinki, Analyt Chem Lab, Dept Chem, FIN-00014 Helsinki, Finland

来源：

JOURNAL OF CHROMATOGRAPHY A | 2011年 / 1218卷 / 27期

基金：

芬兰科学院;

关键词：

Asymmetrical flow field-flow fractionation; Biopolymers; Bioparticles; Macromolecules; Separation; Characterization; ANGLE LIGHT-SCATTERING; VIRUS-LIKE PARTICLES; TRANSMISSION ELECTRON-MICROSCOPY; SIZE-EXCLUSION CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; CAPILLARY-ELECTROPHORESIS; FRIT INLET; PROTEIN AGGREGATION; PROTEOMIC ANALYSIS;

D O I：

10.1016/j.chroma.2010.12.110

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Field-flow fractionation (FFF) is one of the most versatile separation techniques in the field of analytical separation sciences, capable of separating macromolecules in the range 10(3)-10(15) g mol(-1) and/or particles with 1 nm-100 mu m in diameter. The most universal and most frequently used FFF technique, flow FFF, includes three types of techniques, namely symmetrical flow FFF, hollow fiber flow FFF, and asymmetrical flow FFF which is most established variant among them. This review provides a brief look at the theoretical background of analyte retention and separation efficiency in FFF, followed by a comprehensive overview of the current status of asymmetrical flow FFF with selected applications in the field of biopolymers and bioparticles. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：4104 / 4116

页数：13

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