Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments

被引：237

作者：

Ansari, Amir Mehdi ^{[1
,2
]}

Ahmed, A. Karim ^{[1
,2
]}

Matsangos, Aerielle E. ^{[1
,2
]}

Lay, Frank ^{[1
,2
]}

Born, Louis J. ^{[1
,2
]}

Marti, Guy ^{[1
,2
]}

Harmon, John W. ^{[1
,2
]}

Sun, Zhaoli ^{[3
]}

机构：

[1] Dept Surg, Baltimore, MD USA

[2] Hendrix Burn Wound Lab, Baltimore, MD USA

[3] Johns Hopkins Univ, Sch Med, Dept Surg, Baltimore, MD 21205 USA

来源：

STEM CELL REVIEWS AND REPORTS | 2016年 / 12卷 / 05期

关键词：

Green fluorescent protein (GFP); Cytotoxicity; Immunogenicity; Reporter gene; In vivo cell tracking; Cell death; GREEN FLUORESCENT PROTEIN; ACTIN-MYOSIN INTERACTIONS; CYTOTOXIC T-LYMPHOCYTE; MARROW-DERIVED CELLS; STEM-CELL; IMMUNE-RESPONSE; LIVER; CYCLOSPORINE; EXPRESSION; RED;

D O I：

10.1007/s12015-016-9670-8

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Green Fluorescent protein (GFP), used as a cellular tag, provides researchers with a valuable method of measuring gene expression and cell tracking. However, there is evidence to suggest that the immunogenicity and cytotoxicity of GFP potentially confounds the interpretation of in vivo experimental data. Studies have shown that GFP expression can deteriorate over time as GFP tagged cells are prone to death. Therefore, the cells that were originally marked with GFP do not survive and cannot be accurately traced over time. This review will present current evidence for the immunogenicity and cytotoxicity of GFP in in vivo studies by characterizing these responses.

引用

页码：553 / 559

页数：7

共 59 条

[51] The enhanced green fluorescent protein (eGFP) is minimally immunogenic in C57BL/6 mice [J].