Callus formation and plant regeneration from cultured embryos of diploid and tetraploid winter ryes (Secale cereale L)

被引:2
作者
Birsin, Melahat Avci [1 ]
Oezgen, Murat [1 ]
机构
[1] Ankara Univ, Fac Agr, Dept Field Crops, TR-06110 Ankara, Turkey
关键词
callus induction; embryo culture; plant regeneration; Secale cereale L; tissue culture;
D O I
10.1007/BF03263265
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ability of immature, mature and endosperm-supported mature embryos of diploid and tetraploid winter ryes (Secale cereale L) was tested to compare the callus induction and plant regeneration. Immature embryos were obtained from field grown rye. Immature embryos were aseptically excised and placed, with the scutellum upwards, on the callus culture medium consisted of Murashige and Skoog (MS) mineral salts supplemented with 2 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D). Mature embryos were aseptically excised the imbibed seeds and placed, scutellum up, on MS medium supplement with 2 mg l(-1) 2,4-D. Endosperm-supported mature embryos were moved slightly (not set free) in the imbibed mature seeds. The seeds with moved embryos were placed furrow downwards in dishes containing 8 mg l(-1) 2,4-D for callus induction. The developed calli and regenerated plant were maintained on hormone free MS medium. Comparison of the responses of the three explants used indicated that endosperm-supported mature embryo was the most useful explant for plant regeneration in both diploid and tetraploid ryes. This is the first report of winter ryes plants having been regenerated from endosperm-supported mature embryos.
引用
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页码:81 / 84
页数:4
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