Investigation of the Effects of the Endogenous Cannabinoid Anandamide on Luminal A Breast Cancer Cell Line MCF-7

被引：7

作者：

Cetin, Idil ^{[1
]}

Topcul, Mehmet ^{[1
]}

机构：

[1] Istanbul Univ, Fac Sci, Dept Biol, TR-34459 Istanbul, Turkey

来源：

CELLULAR AND MOLECULAR BIOLOGY | 2022年 / 68卷 / 04期

关键词：

Endogenous Cannabinoid; Anandamide MCF-7 In vitro; INDUCED APOPTOSIS; ACTIVATION; BINDS;

D O I：

10.14715/cmb/2022.68.4.16

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was carried out to investigate anti-tumoral effects of Anandamide (AEA) in luminal A breast cancer cell line MCF-7. Cell viability was measured by MTT assay and cell index was measured by xCelligence DP analyzer system. The Feulgen method was used to determine the mitotic index parameter, and the 3H-Thymidine method was used to determine the labeling index parameter. The apoptotic index parameter was determined using a fluorescent dye DAPI. The results of this study showed that 25 mu M Anandamide concentration was the optimum concentration for MCF-7 cells. While this concentration decreased the proportion of cells in the mitotic phase and synthesis phase, it increased the proportion of apoptotic cells.

引用

页码：129 / 133

页数：5

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