Silencing of genes flanking the P1 plasmid centromere

被引：205
|
作者：
Rodionov, O [1 ]
Lobocka, M [1 ]
Yarmolinsky, M [1 ]
机构：
[1] NCI, Biochem Lab, Bethesda, MD 20892 USA
来源：
SCIENCE | 1999年 / 283卷 / 5401期
关键词：
D O I：
10.1126/science.283.5401.546
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Partition modules stabilize bacterial plasmids and chromosomes by actively promoting their segregation into daughter cells. The partition module of plasmid P1 is typical and consists of a centromere site, parS, and genes that encode proteins ParA and ParB. We show that ParB can silence genes flanking parS (to which ParB binds), apparently by polymerizing along the DNA from a nucleation site at parS. Wild-type ParB contacts an extensive region of P1 DNA; silencing-defective ParB proteins, which were found to be partition-defective, are Less able to spread. Hence, the silenced structure appears to function in partitioning.
引用
收藏
页码：546 / 549
页数：4
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