Single-Cellular Biological Effects of Cholesterol-Catabolic Bile Acid-Based Nano/Micro Capsules as Anti-Inflammatory Cell Protective Systems

被引：15

作者：

Mooranian, Armin ^{[1
,2
,3
]}

Ionescu, Corina Mihaela ^{[1
,2
,3
]}

Walker, Daniel ^{[1
,2
,3
]}

Jones, Melissa ^{[1
,2
,3
]}

Wagle, Susbin Raj ^{[1
,2
,3
]}

Kovacevic, Bozica ^{[1
,2
,3
]}

Chester, Jacqueline ^{[1
,2
,3
]}

Foster, Thomas ^{[1
,2
,3
]}

Johnston, Edan ^{[1
,2
,3
]}

Kuthubutheen, Jafri ^{[4
]}

Brown, Daniel ^{[2
,5
]}

Atlas, Marcus D. ^{[3
]}

Mikov, Momir ^{[6
]}

Al-Salami, Hani ^{[1
,2
,3
,7
,8
]}

机构：

[1] Curtin Univ, Curtin Med Sch, Biotechnol & Drug Dev Res Lab, Perth, WA 6102, Australia

[2] Curtin Univ, Curtin Hlth Innovat Res Inst, Perth, WA 6102, Australia

[3] Queen Elizabeth II Med Ctr, Ear Sci Inst Australia, Hearing Therapeut, Perth, WA 6009, Australia

[4] Fiona Stanley Hosp, Murdoch, WA 6150, Australia

[5] Curtin Univ, Curtin Med Sch, Perth, WA 6102, Australia

[6] Univ Novi Sad, Fac Med, Dept Pharmacol Toxicol & Clin Pharmacol, Novi Sad 21101, Serbia

[7] Curtin Univ, Hearing Therapeut Biotechnol & Pharmaceut Sci, Bentley, WA, Australia

[8] Perth QE II Med Centre, Ear Sci Inst Australia, Perth, WA 6009, Australia

来源：

BIOMOLECULES | 2022年 / 12卷 / 01期

关键词：

chenodeoxycholic acid; microencapsulation; inflammation; interleukins; cytokines; EUDRAGIT-RL; BETA-CELLS; CHENODEOXYCHOLIC ACID; DELIVERY; MICROCAPSULES; IMPACT; FLOW; NANOPARTICLES; PERMEABILITY; GLICLAZIDE;

D O I：

10.3390/biom12010073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies in our laboratories have shown promising effects of bile acids in drug encapsulation for oral targeted delivery (via capsule stabilization) particularly when encapsulated with Eudragit NM30D(R) and viable-cell encapsulation and delivery (via supporting cell viability and biological activities, postencapsulation). Accordingly, this study aimed to investigate applications of bile acid-Eudragit NM30D(R) capsules in viable-cell encapsulation ready for delivery. Mouse-cloned pancreatic beta-cell line was cultured and cells encapsulated using bile acid-Eudragit NM30D(R) capsules, and capsules' images, viability, inflammation, and bioenergetics of encapsulated cells assessed. The capsules' thermal and chemical stability assays were also assessed to ascertain an association between capsules' stability and cellular biological activities. Bile acid-Eudragit NM30D(R) capsules showed improved cell viability (e.g., F1 < F2 & F8; p < 0.05), insulin, inflammatory profile, and bioenergetics as well as thermal and chemical stability, compared with control. These effects were formulation-dependent and suggest, overall, that changes in ratios of bile acids to Eudragit NM30D(R) can change the microenvironment of the capsules and subsequent cellular biological activities.

引用

页数：13