Efficient Direct Extended-Spectrum β-Lactamase Detection by Multiplex Real-Time PCR: Accurate Assignment of Phenotype by Use of a Limited Set of Genetic Markers

被引：35

作者：

Ellem, Justin ^{[2
]}

Partridge, Sally R. ^{[1
]}

Iredell, Jonathan R. ^{[1
,2
]}

机构：

[1] Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia

[2] Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 08期

关键词：

KLEBSIELLA-PNEUMONIAE;

D O I：

10.1128/JCM.02647-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum beta-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae.

引用

页码：3074 / 3077

页数：4

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