Efficient Direct Extended-Spectrum β-Lactamase Detection by Multiplex Real-Time PCR: Accurate Assignment of Phenotype by Use of a Limited Set of Genetic Markers

被引：35
作者：
Ellem, Justin [2 ]
Partridge, Sally R. [1 ]
Iredell, Jonathan R. [1 ,2 ]
机构：
[1] Univ Sydney, Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia
[2] Westmead Hosp, Ctr Infect Dis & Microbiol Lab Serv, Westmead, NSW 2145, Australia
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 08期
关键词：
KLEBSIELLA-PNEUMONIAE;
D O I：
10.1128/JCM.02647-10
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum beta-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae.
引用
收藏
页码：3074 / 3077
页数：4
相关论文
共 17 条
  • [1] [Anonymous], 2010, CLSI Document M100-S20
  • [2] The CTX-M β-lactamase pandemic
    Canton, Rafael
    Coque, Teresa M.
    [J]. CURRENT OPINION IN MICROBIOLOGY, 2006, 9 (05) : 466 - 475
  • [3] Multiplex PCR for rapid detection of genes encoding acquired metallo-β-lactamases
    Ellington, Matthew J.
    Kistler, James
    Livermore, David M.
    Woodford, Neil
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 59 (02) : 321 - 322
  • [4] blaIMP-4 in different genetic contexts in Enterobacterzaceae isolates from Australia
    Espedido, Bjoern A.
    Partridge, Sally R.
    Iredell, Jonathan R.
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (08) : 2984 - 2987
  • [5] Metallo-β-lactamase or extended-spectrum β-lactamase:: a wolf in sheep's clothing
    Espedido, Bjoern A.
    Thomas, Lee C.
    Iredell, Jonathan R.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) : 2034 - 2036
  • [6] Clinical and economic impact of common multidrug-resistant gram-negative bacilli
    Giske, Christian G.
    Monnet, Dominique L.
    Cars, Otto
    Carmeli, Yehuda
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (03) : 813 - 821
  • [7] Kasiakou SK., 2005, BMC INFECT DIS, V5, P24, DOI DOI 10.1186/1471-2334-5-24
  • [8] New Disturbing Trend in Antimicrobial Resistance of Gram-Negative Pathogens
    Lee, Jung Hun
    Jeong, Seok Hoon
    Cha, Sun-Shin
    Lee, Sang Hee
    [J]. PLOS PATHOGENS, 2009, 5 (03)
  • [9] International prospective study Klebsiella pneumoniae bacteremia:: Implications of extended-spectrum β-lactamase production in nosocomial infections
    Paterson, DL
    Ko, WC
    Von Gottberg, A
    Mohapatra, S
    Casellas, JM
    Goossens, H
    Mulazimoglu, L
    Trenholme, G
    Klugman, KP
    Bonomo, RA
    Rice, LB
    Wagener, MM
    McCormack, JG
    Yu, VL
    [J]. ANNALS OF INTERNAL MEDICINE, 2004, 140 (01) : 26 - 32
  • [10] Editorial response:: Extended-spectrum β-lactamases:: A call for improved detection and control
    Paterson, DL
    Yu, VL
    [J]. CLINICAL INFECTIOUS DISEASES, 1999, 29 (06) : 1419 - 1422
12