OBSERVATION OF THE ANTIBACTERIAL ACTIVITY AND TIME-KILL KINETICS OF SOME POLYPORES MACROFUNGI EXTRACTS

Natural products produce compounds having the potential of being used against various diseases. It is important to investigate new antimicrobial compounds as a reason for the widespread drug resistance. In this study, the antibacterial potentials of three macrofungi extracts, namely Fomes fomentarius (L.) Fr., Phellinus hartigii (Allesch. & Schnabl) Pat., and Fomitopsis pinicola (Sw.) P. Karst., which were extracted by using six different solvents (water, methanol, chloroform, acetone, petroleum ether, and n-hexane), and examined against twelve different Escherichia coli strains, where one of these strains was a standard strain (E. coli ATCC 25922) and eleven strain were clinical isolates presenting different resistance profiles. The antibacterial activity of the extracts was determined by using minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) tests. In addition, a time-kill kinetics test was conducted for selected extracts. As a result, it was observed that extracts have either bacteriostatic or bacteriocidal activity against E. coli strains. Further research should be done to reveal the chemical composition of the extracts and their mode of action.