Immobilization of polyphenol oxidase in conducting copolymers and determination of phenolic compounds in wines with enzyme electrodes

被引：46

作者：

Kiralp, S

Toppare, L ^{[1
]}

Yagci, Y

机构：

[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

[2] Istanbul Tech Univ, Dept Chem, TR-80626 Istanbul, Turkey

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2003年 / 33卷 / 1-3期

关键词：

polyphenol oxidase; immobilization; electrochemistry; phenolic determination; wine;

D O I：

10.1016/S0141-8130(03)00062-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyphenol oxidase (PPO) was immobilized in copolymers of thiophene functionalized menthyl monomer (MM) with pyrrole. Immobilization of enzyme was performed via entrapment in conducting copolymers during electrochemical polymerization of pyrrole. Maximum reaction rates. Michaelis-Menten constants and temperature, pH and operational stabilities of enzyme electrodes were investigated. Total amount of phenolic compounds in red wines of Turkey were analyzed by using these electrodes. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：37 / 41

页数：5

共 18 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] Identification of novel polyphenol oxidase inhibitors by enzymatic one-pot synthesis and deconvolution of combinatorial libraries [J].