Chemical force microscopy with active enzymes

被引:27
|
作者
Fiorini, M [1 ]
McKendry, R [1 ]
Cooper, MA [1 ]
Rayment, T [1 ]
Abell, C [1 ]
机构
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
关键词
D O I
10.1016/S0006-3495(01)76215-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with competitive binding of other ligands in solution show that the observed adhesion forces arise predominantly from specific interactions between the immobilized enzyme and surface-bound adenine derivative. These experiments represent a step in the development of a screening methodology based upon chemical force microscopy.
引用
收藏
页码:2471 / 2476
页数:6
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