Troponin Aptamer on an Atomically Flat Au Nanoplate Platform for Detection of Cardiac Troponin I

被引：29

作者：

Lee, Hyoban ^{[1
]}

Youn, Hyungjun ^{[2
]}

Hwang, Ahreum ^{[1
,3
]}

Lee, Hyunsoo ^{[1
,4
]}

Park, Jeong Young ^{[1
,4
]}

Kim, Weon ^{[5
]}

Yoo, Youngdong ^{[6
]}

Ban, Changill ^{[2
]}

Kang, Taejoon ^{[3
]}

Kim, Bongsoo ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea

[2] POSTECH, Dept Chem, Pohang 37673, South Korea

[3] KRIBB, Bionanotechnol Res Ctr, Daejeon 34141, South Korea

[4] Inst for Basic Sci Korea, Ctr Nanomat & Chem React, Daejeon 34141, South Korea

[5] Kyung Hee Univ, Kyung Hee Univ Hosp, Dept Internal Med, Div Cardiol, Seoul 02447, South Korea

[6] Ajou Univ, Dept Chem, Suwon 16499, South Korea

来源：

NANOMATERIALS | 2020年 / 10卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

cTnI; aptamer; nanoplate; surface-enhanced Raman scattering; EARLY-DIAGNOSIS; IMMUNOASSAY;

D O I：

10.3390/nano10071402

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Well-ordered bioreceptors on atomically flat Au surfaces can be a high-performance biosensor. Cardiac troponin I proteins (cTnIs) have been regarded as a specific biomarker for acute myocardial infarction (AMI). Here, we report the accurate detection of cTnIs using an aptamer-immobilized Au nanoplate platform. The single-crystalline and atomically flat Au nanoplate was characterized by atomic force microscopy. For the precise detection of cTnI, we immobilized an aptamer that can strongly bind to cTnI onto an atomically flat Au nanoplate. Using the aptamer-immobilized Au nanoplate, cTnIs were successfully detected at a concentration of 100 aM (2.4 fg/mL) in buffer solution. Furthermore, cTnIs in serum could be identified at a concentration of 100 fM (2.4 pg/mL). The total assay time was similar to 7 h. Importantly, the aptamer-immobilized Au nanoplate enabled us to diagnose AMI patients accurately, suggesting the potential of the present method in the diagnosis of AMI.

引用

页码：1 / 10

页数：10

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