An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

被引：15

作者：

Hasenkamp, Sandra ^{[1
]}

Wong, Eleanor H. ^{[2
]}

Horrocks, Paul ^{[1
]}

机构：

[1] Keele Univ, Inst Sci & Technol Med, Keele ST5 5BG, Staffs, England

[2] Glasgow Biomed Res Ctr, Wellcome Ctr Mol Parasitol, Glasgow G12 8QQ, Lanark, Scotland

来源：

MALARIA JOURNAL | 2012年 / 11卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

Luciferase; Single-step lysis; Stage-specific; Promoter function; pcna; EXPRESSION; ASSAY;

D O I：

10.1186/1475-2875-11-42

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

引用

页数：7

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