A radioanalytical technique for measurement of beta-glucuronidase activities

被引:4
作者
Ünak, T [1 ]
Avcibasi, U [1 ]
Yildirim, Y [1 ]
机构
[1] Ege Univ, Fac Sci, Dept Chem, Div Nucl Chem, TR-35100 Izmir, Turkey
关键词
D O I
10.1007/s10967-005-0939-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Phenolphthalein-glucuronide is a commonly used glucuronide conjugate for beta-glucuronidase measurements. The quantity of phenolphthalein liberated by beta-glucuronidase is measured spectrophotometrically. The detection limit of the quantity of phenolphthalein using spectrophotometry is a few mu g. In this study, a new radioanalytical technique for the measurement of beta-glucuronidase was applied which is 10(6) times more sensitive than the spectrophotometric technique. Radioiodinated phenolphthalein-glucuronide and phenyl-N-glucuronide were used in this study in which the beta-glucuronidase levels of some tissue samples were measured.
引用
收藏
页码:503 / 506
页数:4
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