Estimating microbial population counts by 'most probable number' using Microsoft Excel®

被引：96

作者：

Briones, AM ^{[1
]}

Reichardt, W ^{[1
]}

机构：

[1] Int Rice Res Inst, Manila 1099, Philippines

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1999年 / 35卷 / 02期

关键词：

computer software; most probable number; microbial populations; counts; Microsoft Excel;

D O I：

10.1016/S0167-7012(98)00111-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A computer-assisted method for determining population counts using the 'most probable number' (MPN) was developed. The Microsoft Excel(R) spreadsheet and its Solver(R) tool were used to generate MPNs, error estimates and confidence limits. Our method was flexible, allowing the use of unbalanced replication schemes and varying replication numbers and inoculation volumes. Furthermore, it required no programming skills and generated fast results, which were comparable to those of standard MPN tables and MPN software. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：157 / 161

页数：5

共 9 条

[1]

[Anonymous], 1982, METHODS SOIL ANAL

[2] ACCURACY OF A PLANT-INFECTION TECHNIQUE FOR COUNTING POPULATIONS OF RHIZOBIUM TRIFOLII [J].