Equivalence of the Huygens-Fresnel and Debye approach for the calculation of high aperture point-spread functions in the presence of refractive index mismatch

被引:44
作者
Egner, A [1 ]
Hell, SW [1 ]
机构
[1] Max Planck Inst Biophys Chem, High Resolut Opt Microscopy Grp, D-37070 Gottingen, Germany
来源
JOURNAL OF MICROSCOPY-OXFORD | 1999年 / 193卷
关键词
aberration; confocal; fluorescence; microscopy; multiphoton; point spread function; refractive index mismatch; resolution;
D O I
10.1046/j.1365-2818.1999.00462.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
As discussed in recent work (Sheppard, C. J. R. & Torok, P., I. Microsc.. 185, 366-384; Torok et al., J. Microsc., 188, 158-172), two approaches have been used extensively for vectorial computations of high aperture confocal point-spread functions when focusing through a dielectric interface. Whereas the equation by Hell, Reiner, Cremer & Stelzer (I. Microsc., 169, 391-405) is based on the Huygens-Fresnel principle, the more recent approach by Torok. Varga Br Booker (J. Opt. Sec. Aln. A, 12, 325-332; J. Opt. SOC. Am. A, 12, 2136-2144) is based on the Debye approximation. While the earlier theory considers a large but finite focal length the second theory is derived for an infinitely high Fresnel number, In a high aperture microscope, a high Fresnel number is equivalent to assuming that the focal length be infinitely large with respect to the wavelength. So far, the two theories are regarded as different, with the one by Torok et al, being rigorous, In this paper, we demonstrate that, if the same conditions are applied, the equation by Torok et ttl, can be analytically derived from that by Hell et nl. Producing the same results, the benefit brought about by the equation by Torok el nl. is improved flexibility and computational speed for cases with azimuthal symmetry.
引用
收藏
页码:244 / 249
页数:6
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