Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy

被引：12
作者：
Liu, Honghai [1 ,2 ]
Qin, Wan [1 ,2 ]
Shao, Yonghong [3 ]
Ma, Zhen [1 ,2 ]
Ye, Tong [4 ]
Borg, Tom [5 ]
Gao, Bruce Z. [1 ,2 ]
机构：
[1] Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA
[2] COMSET, Clemson, SC 29634 USA
[3] Shenzhen Univ, Inst Optoelect, Shenzhen 518060, Guangdong, Peoples R China
[4] Univ Alabama Birmingham, Dept Neurobiol, Birmingham, AL 35294 USA
[5] Med Univ S Carolina, Dept Regenerat Med & Cell Biol, Charleston, SC 29425 USA
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2011年 / 16卷 / 12期
基金：
中国国家自然科学基金;
关键词：
two-photon excitation fluorescence; second harmonic generation; on-stage incubator; cardiomyocytes; myofibrillogenesis; sarcomere addition; SARCOMERE-LENGTH;
D O I：
10.1117/1.3662457
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3662457]
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页数：4
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