Modulation of Toll-like receptor-mediated innate immunity in bovine intestinal epithelial cells by lactic acid bacteria isolated from feedlot cattle

被引：14

作者：

Mansilla, F. ^{[1
]}

Takagi, M. ^{[2
]}

Garcia-Castillo, V ^{[1
,2
]}

Aso, H. ^{[3
,4
]}

Nader-Macias, M. E. ^{[1
]}

Vignolo, G. ^{[1
]}

Kitazawa, H. ^{[2
,4
]}

Villena, J. ^{[1
,2
]}

机构：

[1] Reference Ctr Lactobacilli CERELA CONICET, Chacabuco 145, RA-4000 San Miguel De Tucuman, Argentina

[2] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Prod Chem, Food & Feed Immunol Grp,Aoba Ku, 468-1 Aramaki Aza Aoba, Sendai, Miyagi 9808572, Japan

[3] Tohoku Univ, Grad Sch Agr Sci, Cell Biol Lab, Aoba Ku, 468-1 Aramaki Aza Aoba, Sendai, Miyagi 9808572, Japan

[4] Tohoku Univ, Int Educ & Res Ctr Food Agr Immunol CFAI, Grad Sch Agr Sci, Livestock Immunol Unit,Aoba Ku, 468-1 Aramaki Aza Aoba, Sendai, Miyagi 9808572, Japan

来源：

BENEFICIAL MICROBES | 2020年 / 11卷 / 03期

基金：

日本学术振兴会;

关键词：

bovine intestinal epithelial cells; immunobiotic; TLR4; TLR3; TLRs negative regulators; Lactobacillus mucosae CRL2069; NF-KAPPA-B; EXPRESSION; RESPONSES; A20; IDENTIFICATION; PHOSPHATASE-1; PATHOGENESIS; ASSOCIATION; PREVENTION; PROBIOTICS;

D O I：

10.3920/BM2019.0189

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ability of lactobacilli isolated from feedlot cattle environment to differentially modulate the innate immune response triggered by Toll-like receptors (TLRs) activation in bovine intestinal epithelial (BIE) cells was evaluated. BIE cells were stimulated with Lactobacillus mucosae CRL2069, Lactobacillus acidophilus CRL2074, Lactobacillus fermentum CRL2085 or Lactobacillus rhamnosus CRL2084 and challenged with heat-stable pathogen associated molecular patterns (PAMPs) from enterotoxigenic Escherichia coli (ETEC) to induce the activation of TLR4 or with polyinosinic:polycytidylic acid (poly(I:C)) to activate TLR3. Type I interferons, cytokines, chemokines and negative regulators of TLR signalling were studied by RT-PCR. L. mucosae CRL2069 significantly reduced the expression of interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 in BIE cells in the context of TLR3 activation. L. mucosae CRL2069 also reduced the expression of tumour necrosis factor-alpha, IL-beta, MCP-1, and IL-8 in heat-stable ETEC PAMPs-challenged BIE cells. In addition, reduced expressions of IL-6, MCP-1, and IL-8 were found in BIE cells stimulated with L. rhamnosus CRL2084, although its effect was significantly lower than that observed for the CRL2069 strain. The reduced levels of pro-inflammatory factors in BIE cells induced by the CRL2069 and CRL2085 strains was related to their ability of increasing the expression of TLR negative regulators. L. mucosae CRL2069 significantly improved the expression of A20-binding inhibitor of NF kappa-B activation 3 (ABIN-3), interleukin-1 receptor-associated kinase M (IRAK-M) and mitogen-activated protein kinase 1 (MKP-1) while L. rhamnosus CRL2084 augmented ABIN-3 expression in BIE cells. The results of this work suggest that among the studied strains, L. mucosae CRL2069 was able to regulate TLR3-mediated innate immune response and showed a remarkable capacity to modulate TLR4-mediated inflammation in BIE cells. The CRL2069 strain induce the upregulation of three TLR negative regulators that would influence nuclear factor kB and mitogen-activated protein kinases signalling pathways while reducing the expression of pro-inflammatory cytokines and chemokines. Therefore, L. mucosae CRL2069 is an interesting immunobiotic candidate for the protection of the bovine host against TLR-mediated intestinal inflammatory damage.

引用

页码：269 / 282

页数：14