Elucidating nitrifying performance, nitrite accumulation and microbial community in a three-stage plug flow moving bed biofilm reactor (PF - MBBR)

被引:21
作者
Fan, Yajun [1 ]
Zhang, Miao [2 ]
Cheng, Jilin [1 ]
Yong, Daming [3 ]
Ji, Junjie [2 ]
Wu, Qichao [3 ]
He, Chengda [2 ]
机构
[1] Yangzhou Univ, Coll Hydraul Sci & Engn, Yangzhou 225100, Jiangsu, Peoples R China
[2] Yangzhou Univ, Coll Environm Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China
[3] Yangzhou Polytech Inst, Yangzhou 225127, Jiangsu, Peoples R China
关键词
PF; MBBR; Biofilm; Nitrification; NO2 -N accumulation; Microbial community structure; MBBR optimization; WASTE-WATER TREATMENT; DENITRIFYING PHOSPHORUS REMOVAL; AMMONIA-OXIDIZING BACTERIA; NITROGEN REMOVAL; SIMULTANEOUS NITRIFICATION; SURFACE MODIFICATION; POPULATION-DYNAMICS; DISSOLVED-OXYGEN; DENITRIFICATION; TEMPERATURE;
D O I
10.1016/j.chemosphere.2022.134087
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A three-stage plug flow moving bed biofilm reactor (PF -MBBR, consisting of three identical chambers of N1, N2 and N3) was proposed for nitrifier enrichment using synthetic wastewater. During the stable operation, the average NH4(+)- N effluent was 0.67 mg/L and NH4(+)-N removal was as high as 97.19% with the nitrite accumulation ratio (NAR) of 54.23%, although the biofilm thickness and biomass both presented downward trends from N1 (296 mu m, 2280 mg/L), N2 (248 mu m, 1850 mg/L) to N3 (198 mu m, 1545 mg/L). Particularly, the comparative results of three stages revealed that N2 showed the optimum NH4(+)-N removal (77.27%) and NAR (75.21%) in the continuous-flow, while NAR of N3 unexpectedly maintained a high level of 65.83% in the batch test, suggesting that ammonia oxidizing bacteria (AOB) accounted for absolute advantage over nitrite oxidizing bacteria (NOB). High-throughput sequencing initially verified different distribution of bacterial community structure, where N2 was far away from N1 and N3 with the lowest community richness and community diversity (operational taxonomic units (OTUs): 454(N2)< 527(N3)< 621(N1)). Proteobacteria (77.60%-83.09%), Bacteroidetes (1.66%-3.66%), Acidobacteria (2.28%-4.67%), and Planctomycetes (1.19%-6.63%) were the major phyla. At the genus level, AOB (mainly Nitrosomonas) accounted for 5.08% (N1), 20.74% (N2) and 14.24% (N3) while NOB (mainlyNitrospira) increased from 0.14% (N1), 7.06% (N2) to 4.91% (N3) with the total percentages of 5.22%, 27.80% and 19.15%. Finally, the application feasibility of MBBR optimization linked with nitrite (NO2--N) accumulation for deep-level nutrient removal was discussed.
引用
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页数:10
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