Cloning and expression of hamster telomerase catalytic subunit cDNA

被引:0
作者
Guo, W
Okamoto, M
Park, NH
Lee, YM
Park, NH
机构
[1] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Dent Res Inst, Los Angeles, CA 90095 USA
关键词
hamster; telomerase; cDNA; cloning; expression;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Topical application of 7,12-dimethylbenz(a)anthracene induces tumors in the hamster cheek pouch. Telomerase activity is increased in the cancer tissues if compared to normal adjacent tissues in both human and hamster oral cancer. In order to achieve a probe and to investigate the putative role of telomerase in oral carcinogenesis using the hamster cheek pouch model, we have cloned the cDNA encoding the hamster telomerase catalytic subunit (hamTERT). The hamster TERT cDNA encoded 1128 amino acids and shared 64% amino acid identity with human TERT and 80% with murine TERT. As noted with human TERT which express several alternatively spliced mRNAs, we have also detected one alternatively spliced hamTERT mRNA in hamster cancer cells. Transient transfection of hamTERT cDNA in a retroviral expression vector reconstituted telomerase activity in the telomerase negative human lung fibroblast IMR90 cells.
引用
收藏
页码:73 / 78
页数:6
相关论文
共 30 条
[1]   TELOMERE SHORTENING IS ASSOCIATED WITH CELL-DIVISION IN-VITRO AND IN-VIVO [J].
ALLSOPP, RC ;
CHANG, E ;
KASHEFIAAZAM, M ;
ROGAEV, EI ;
PIATYSZEK, MA ;
SHAY, JW ;
HARLEY, CB .
EXPERIMENTAL CELL RESEARCH, 1995, 220 (01) :194-200
[2]  
Avilion AA, 1996, CANCER RES, V56, P645
[3]   TELOMERES [J].
BLACKBURN, EH .
TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (10) :378-381
[4]   Extension of life-span by introduction of telomerase into normal human cells [J].
Bodnar, AG ;
Ouellette, M ;
Frolkis, M ;
Holt, SE ;
Chiu, CP ;
Morin, GB ;
Harley, CB ;
Shay, JW ;
Lichtsteiner, S ;
Wright, WE .
SCIENCE, 1998, 279 (5349) :349-352
[5]   Secondary structure of vertebrate telomerase RNA [J].
Chen, JL ;
Blasco, MA ;
Greider, CW .
CELL, 2000, 100 (05) :503-514
[6]   THE RNA COMPONENT OF HUMAN TELOMERASE [J].
FENG, JL ;
FUNK, WD ;
WANG, SS ;
WEINRICH, SL ;
AVILION, AA ;
CHIU, CP ;
ADAMS, RR ;
CHANG, E ;
ALLSOPP, RC ;
YU, JH ;
LE, SY ;
WEST, MD ;
HARLEY, CB ;
ANDREWS, WH ;
GREIDER, CW ;
VILLEPONTEAU, B .
SCIENCE, 1995, 269 (5228) :1236-1241
[7]   Telomere length regulation [J].
Greider, CW .
ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 :337-365
[8]   Enhanced activity of cloned hamster TERT gene promoter in transformed cells [J].
Guo, WT ;
Okamoto, M ;
Lee, YM ;
Baluda, MA ;
Park, NH .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2001, 1517 (03) :398-409
[9]  
Guo WT, 1999, INT J ONCOL, V15, P817
[10]   Ethanol upregulates the expression of p21(WAF1/CIP1) and prolongs G(1) transition via a p53-independent pathway in human epithelial cells [J].
Guo, WT ;
Baluda, MA ;
Park, NH .
ONCOGENE, 1997, 15 (10) :1143-1149