Changes in oxygen sensitivity of TASK in carotid body glomus cells during early postnatal development

被引:17
作者
Kim, Donghee [1 ]
Papreck, Justin R. [1 ]
Kim, Insook [2 ]
Donnelly, David F. [3 ]
Carroll, John L. [2 ]
机构
[1] Rosalind Franklin Univ Med & Sci, Dept Physiol & Biophys, N Chicago, IL 60064 USA
[2] Univ Arkansas Med Sci, Arkansas Childrens Hosp, Dept Pediat, Div Pediat Pulm Med, Little Rock, AR 72202 USA
[3] Yale Univ, Dept Pediat, Sch Med, Sect Resp Med, New Haven, CT 06520 USA
关键词
Carotid body; Chemoreceptor; Potassium channel; Oxygen sensing; Development; ARTERIAL CHEMORECEPTOR CELLS; K+ CHANNEL; I CELLS; RAT; HYPOXIA; MATURATION; CHEMOSENSITIVITY; TASK-1/TASK-3; EXPRESSION; RESPONSES;
D O I
10.1016/j.resp.2011.04.012
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
A post-natal increase in carotid body (CB) hypoxia responsiveness occurs at the level of carotid sinus nerve activity, intracellular calcium, cell membrane depolarization and hypoxic inhibition of O-2-sensitive background K+ conductance. TASK-1, TASK-1/3 and TASK-3 are functionally expressed in CB glomus cells, with TASK-1/3 providing the major part of the O-2-sensitive TASK-like background K+ conductance. Here we report the effects of graded hypoxia on TASK-like channel activity in CB glomus cells from rats aged 0 to 1,6 to 7 and 16 to 18 days: the time frame of postnatal CB functional maturation. TASK was active in nearly all cell-attached patches and TASK activity during normoxia did not differ across ages. Hypoxia produced a progressive decrease in channel opening frequency with graded decreases in O-2 level and also produced glomus cell depolarization, as assessed by the shift in reversal potential of TASK single channel current. Hypoxic inhibition of TASK activity was least at P0-P1 and increased with age mainly between 6-7 and 16-18 days. The O-2-sensitive TASK activity was significantly greater in glomus cells from P16 to P18 when compared to cells from PO to P1 day old rats. These results support the hypothesis that postnatal carotid body functional maturation is due, at least in part, to changes in the sensitivity of TASK to the hypoxic signals generated in glomus cells. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:228 / 235
页数:8
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