Impaired antigen specific responses and enhanced polyclonal stimulation in mice infected with Ehrlichia muris

The immune status of BALB/c mice infected by intraperitoneal inoculation with Ehrlichia muris was examined, The level off. muris infection in both peritoneal cavity and spleen was greatest at day 10 postinoculation (PI), Thereafter, the infection level was dramatically reduced while the organism persisted for up to 400 days PI. The greatest intraperitoneal infiltration of leukocytes, splenomegaly, and leukocytosis were observed on days 10, 15, and 20 PI, respectively, Infected mice developed marked hypergammaglobulinemia of IgG and IgM that peaked at day 20 PI; however, IgA plummeted at day 15 PI, Of IgG, G2a and G3 increased while G1 and G2b remained constant, Despite hypergammaglobulinemia, both IgG and IgM antibody titers against E. muris were very low throughout the 30-day study, Antibody development and plaque-forming cells against sheep red blood cells (SRBC) were abolished when the antigen was inoculated on day 10 PI, IgM antibody development against SRBC was more severely inhibited than IgG antibody development, However, when mice were immunized with SRBC prior to E. muris infection, antibody development against SRBC was not reduced, Delayed type hypersensitivity reaction to dinitrofluorobenzene was also maximally inhibited when the antigen was administered on day 10 PI, The IFN-gamma level in the blood was maximal at day 10 PI, These results indicate that although the vigorous polyclonal activation and protective IFN-gamma responses occurred by day 10 PI - which cleared most of the ehrlichial infection - antigen-specific immune stimulation was impaired primarily at the level of antigen-priming at peak parasitemia.