Live Cell MicroRNA Imaging Using Cascade Hybridization Reaction

被引：220
作者：
Cheglakov, Zoya [2 ,3 ]
Cronin, Timothy M. [1 ]
He, Chuan [2 ,3 ]
Weizmann, Yossi [1 ]
机构：
[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[3] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 19期
关键词：
IN-SITU HYBRIDIZATION; FLUORESCENT-PROBES; MOLECULAR BEACONS; GENE-EXPRESSION; RNA; VISUALIZATION; IDENTIFICATION; 2'-O-METHYL; DESIGN;
D O I：
10.1021/jacs.5b01451
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Recent advances in RNA research have posed new directives in biology and chemistry to uncover the complex roles of ribonucleic acids in cellular processes. Innovative techniques to visualize native RNAs, particularly, short, low-abundance RNAs in live cells, can dramatically impact current research on the rotes of RNAs in biology. Herein, we report a novel method for real-time, microRNA imaging inside live cells based on programmable oligonucleotide probes, which self-assemble through the Cascade Hybridization Reaction (CHR).
引用
收藏
页码：6116 / 6119
页数：4
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