Live Cell MicroRNA Imaging Using Cascade Hybridization Reaction

被引：215
作者：
Cheglakov, Zoya [2 ,3 ]
Cronin, Timothy M. [1 ]
He, Chuan [2 ,3 ]
Weizmann, Yossi [1 ]
机构：
[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[3] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2015年 / 137卷 / 19期
关键词：
IN-SITU HYBRIDIZATION; FLUORESCENT-PROBES; MOLECULAR BEACONS; GENE-EXPRESSION; RNA; VISUALIZATION; IDENTIFICATION; 2'-O-METHYL; DESIGN;
D O I：
10.1021/jacs.5b01451
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Recent advances in RNA research have posed new directives in biology and chemistry to uncover the complex roles of ribonucleic acids in cellular processes. Innovative techniques to visualize native RNAs, particularly, short, low-abundance RNAs in live cells, can dramatically impact current research on the rotes of RNAs in biology. Herein, we report a novel method for real-time, microRNA imaging inside live cells based on programmable oligonucleotide probes, which self-assemble through the Cascade Hybridization Reaction (CHR).
引用
收藏
页码：6116 / 6119
页数：4
相关论文
共 28 条
  • [1] Alahar S., 2013, MICRORNA CANC, VVIII, P148
  • [2] Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques
    Amann, Rudolf
    Fuchs, Bernhard M.
    [J]. NATURE REVIEWS MICROBIOLOGY, 2008, 6 (05) : 339 - 348
  • [3] Fluorescent Probes for Live-Cell RNA Detection
    Bao, Gang
    Rhee, Won Jong
    Tsourkas, Andrew
    [J]. ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2009, 11 : 25 - 47
  • [4] Fluorescent Probes for Nucleic Acid Visualization in Fixed and Live Cells
    Boutorine, Alexandre S.
    Novopashina, Darya S.
    Krasheninina, Olga A.
    Nozeret, Karine
    Venyaminova, Alya G.
    [J]. MOLECULES, 2013, 18 (12) : 15357 - 15397
  • [5] Programmable in situ amplification for multiplexed imaging of mRNA expression
    Choi, Harry M. T.
    Chang, Joann Y.
    Trinh, Le A.
    Padilla, Jennifer E.
    Fraser, Scott E.
    Pierce, Niles A.
    [J]. NATURE BIOTECHNOLOGY, 2010, 28 (11) : 1208 - U103
  • [6] FISHing for chick genes:: Triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos
    Denkers, N
    García-Villalba, P
    Rodesch, CK
    Nielson, KR
    Mauch, TJ
    [J]. DEVELOPMENTAL DYNAMICS, 2004, 229 (03) : 651 - 657
  • [7] Molecular beacon DNA probes and their bioanalytical applications
    Drake, TJ
    Tan, WH
    [J]. APPLIED SPECTROSCOPY, 2004, 58 (09) : 269A - 280A
  • [8] Crystallographic Studies of Chemically Modified Nucleic Acids: A Backward Glance
    Egli, Martin
    Pallan, Pradeep S.
    [J]. CHEMISTRY & BIODIVERSITY, 2010, 7 (01) : 60 - 89
  • [9] Rational design of DNA nanoarchitectures
    Feldkamp, U
    Niemeyer, CM
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (12) : 1856 - 1876
  • [10] Rational engineering of dynamic DNA systems
    Feldkamp, Udo
    Niemeyer, Christof M.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (21) : 3871 - 3873
123