Escherichia coli Dam-methylase as a molecular tool for mapping binding sites of the yeast transcription factor Rpn4

被引：8
作者：
Spasskaya, D. S. [1 ]
Karpov, D. S. [1 ]
Karpov, V. L. [1 ]
机构：
[1] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia
来源：
MOLECULAR BIOLOGY | 2011年 / 45卷 / 04期
基金：
俄罗斯基础研究基金会;
关键词：
Rpn4; Saccharomyces cerevisiae; Escherichia coli; Dam-methylase; binding site; SACCHAROMYCES-CEREVISIAE; IN-VIVO; FEEDBACK-CIRCUIT; SHUTTLE VECTORS; EXCISION-REPAIR; BUDDING YEAST; PROTEASOME; GENES; IDENTIFICATION; METHYLTRANSFERASE;
D O I：
10.1134/S0026893311030186
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Rpn4p is a transcription factor responsible for coordinated regulation of proteasomal genes in Saccharomyces cerevisiae. There are data suggesting an involvement of this factor in regulation of many other genes that comprise more than one tenth part of the yeast genome. Traditional methods are inapplicable for mapping of Rpn4p binding sites because of their extremely low level. We have developed a model system using Dam-methylase of E. coli which allows us to detect interaction of Rpn4p with its target genes. In this system, we have shown that Rpn4p is recruited to proteasomal genes only through interactions with DNA.
引用
收藏
页码：591 / 599
页数：9
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